Anti-Lipoprotein a 抗体 (Biotin) (ab27631)

製品の概要

  • 製品名Anti-Lipoprotein a antibody (Biotin)
    Lipoprotein a 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to Lipoprotein a (Biotin)
  • 標識Biotin
  • 特異性Thsi antibody specifically binds to human Lipoprotein a.
  • アプリケーション適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native Lipoprotein a protein (purified) (Human)

  • ポジティブ・コントロール
    • This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab27631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/20000.
ELISA 1/5000 - 1/20000.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 関連性Lipoprotein(a) (Lp(a)) is a lipoprotein subclass assembled in the blood from low density lipoprotein (LDL) molecules and apolipoprotein-a (apo-a). Lp(a) recruits inflammatory cells through interaction with Mac-1 integrin. High Lp(a) in blood is a risk factor for coronary heart disease, cerebrovascular disease, atherosclerosis, thrombosis, and stroke. Lp(a) concentrations may be affected by disease states, but are only moderately affected by diet, exercise and other environmental factors. Lipid-reducing drugs have no effect on Lp(a) concentration. High Lp(a) predicts risk of early atherosclerosis similar to high LDL, but in advanced atherosclerosis, Lp(a) is a risk factor independent of LDL, indicating a coagulant risk of plaque thrombosis. Apo(a) contains domains that are very similar to plasminogen (PLG). Lp(a) accumulates in the vessel wall and inhibits binding of PLG to the cell surface, reducing plasmin generation which increases clotting. This inhibition also promotes proliferation of smooth muscle cells. These unique features of Lp(a) suggest a role in the generation of clots and atherosclerosis.
  • 参照データベース
  • 別名
    • AK38 antibody
    • Antiangiogenic AK38 protein antibody
    • apo(a) antibody
    • APOA antibody
    • apolipoprotein(a) antibody
    • LP antibody
    • lp(a) antibody
    • LPA antibody
    see all

Anti-Lipoprotein a antibody (Biotin) 画像

  • ICC/IF image of ab27631 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab27631 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- Sheep (ab96939) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Lipoprotein a antibody (Biotin) (ab27631) 使用論文

ab27631 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization No
Specification Liver
Blocking step Casein as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Formaldehyde
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投稿 Oct 13 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization No
Specification Liver
Blocking step Casein as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 13 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"