製品の概要

  • 製品名Anti-Lipin 1 antibody
    Lipin 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Lipin 1
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae, Zebrafish
  • 免疫原

    Synthetic peptide: SLAVIYPQSA SYPNSDREWS PTPSPSGSRP STPKSDSELV SKSTERTGQK, corresponding to amino acids 218-267 of Human Lipin 1 (NP_663731)

  • ポジティブ・コントロール
    • 721_B cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 99 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Plays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol.
  • 組織特異性Abundant in adipose tissue and skeletal muscle. Lower levels in some portions of the digestive tract.
  • 関連疾患Defects in LPIN1 are a cause of autosomal recessive acute recurrent myoglobinuria (ARARM) [MIM:268200]; also known as acute recurrent rhabdomyolysis. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness and followed by excretion of myoglobin in the urine. Renal failure may occasionally occur. Onset is usually in early childhood under the age of 5 years.
  • 配列類似性Belongs to the lipin family.
  • ドメインContains one Leu-Xaa-Xaa-Ile-Leu (LXXIL), a transcriptional binding motif, which mediates interaction with PPARA.
    Contains 1 Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif essential for phosphatidate phosphatase activity.
  • 翻訳後修飾Phosphorylated at multiple sites in response to insulin. Phosphorylation is controlled by the mTOR signaling pathway (By similarity). Dephosphorylated in response to epinephrine and oleic acid.
    Sumoylated.
  • 細胞内局在Nucleus. Cytoplasm > cytosol. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC=3.1.3.4 antibody
    • KIAA0188 antibody
    • Lipin-1 antibody
    • Lpin1 antibody
    • LPIN1_HUMAN antibody
    • PAP1 antibody
    • Phosphatidate phosphatase LPIN1 antibody
    see all

Anti-Lipin 1 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling Lipin 1 with ab84950 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm and membrane of cell bodies and processes of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Lipin 1. Right - Merge.
  • Anti-Lipin 1 antibody (ab84950) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 99 kDa
    Observed band size : >90 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab84950 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84950, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Lipin 1 antibody (ab84950) 使用論文

ab84950 has not yet been referenced specifically in any publications.

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