Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) (ab118970)

製品の概要

  • 製品名Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric)
  • 検出方法Colorimetric/Fluorometric
  • テスト
    100 x 1 assay
  • サンプルの種類
    Plasma, Cell culture extracts, Tissue Extracts
  • アッセイタイプQuantitative
  • 検出感度
    > 0.1 nmol/well
  • 製品の概要

    Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) (ab118970) provides a convenient tool for sensitive detection of the malondialdehyde (MDA) present in a variety of samples. MDA, together with 4-hydroxynonenal (4-HNE), is a natural bi-product of lipid peroxidation and its quantification is generally used as marker for lipid peroxidation. The MDA in the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.



    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Lipid peroxidation refers to the oxidative degradation of lipids. In this process free radicals take electrons from the lipids (generally in cell membranes), resulting in cell damage. Quantification of lipid peroxidation is essential to assess oxidative stress in pathophysiological processes. Lipid peroxidation forms reactive aldehydes such as malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE) as natural bi-products. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage.

  • アプリケーション適用あり: Functional Studiesmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Lipid peroxidation refers to the oxidative degradation of lipids and is a well-defined mechanism of cellular damage. The formation of lipid peroxidation products leads to spread of free radical reactions leading to cell damage.

アプリケーション

Our Abpromise guarantee covers the use of ab118970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) 画像

  • Typical MDA standard calibration curve using colorimetric reading.

  • Typical MDA standard calibration curve using fluorometric reading.

  • Measurement of MDA in human plasma (20 µl) and rat liver lysate (10 mg).

プロトコール

Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) (ab118970) 使用論文

This product has been referenced in:
  • Qiao YF  et al. Melatonin attenuates hypertension-induced renal injury partially through inhibiting oxidative stress in rats. Mol Med Rep 13:21-6 (2016). Read more (PubMed: 26531807) »
  • Wang F  et al. Antithrombin III/SerpinC1 insufficiency exacerbates renal ischemia/reperfusion injury. Kidney Int 88:796-803 (2015). Read more (PubMed: 26108065) »

See all 9 Publications for this product

Product Wall


I confirmed with the laboratories that typically we do not recommend RIPA buffer for these kits since it contains SDS which can denature proteins.
We suggest to use the lysis buffer that comes with the ab118970 Lipid Peroxidation (MDA) Assay ...

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Yes, heparin should be fine to use with ab65328 and ab118970.

Below please find answers to your questions:

A1: We have found that the 10 ul plasma worked well. How much more plasma is used depends on the protein content.
A2: If there is low amounts of MDA adduct formed in plasma samples, then the flu...

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I double checked and unfortunately, have confirm that we do not have a kit for measuring NADPH oxidase in our catalog at the moment. We do offer ab65349 which is a NADP/NADPH Assay Kit:

http://www.abcam.com/index.html?datasheet=6534...

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Centrifuge the samples and take only the clear supernatant for the next step.

Thank you for your enquiry.

I can confirm that you will need to use the n-butanol for the standards as well, since it helps in isolating the MDA-TBA adduct.

I hope this information has been useful for you. Please do not hesitate t...

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Thank you for contacting us about this issue. The lab says the TBA generally goes into solution after mixing the TBA in acetic acid with the water. Did you see any apparent dissolving after mixing the two by vortexing? Did you try heating it in a fume ...

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Regarding ab133085, we do not have data for the collection, freezing and storage of plant tissue samples for 6 months at -80ºC. From your customer’s protocol, they did a good job of collecting, freezing and storing their tissues, but th...

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Thank you for your inquiry.

I heard from the lab that you can start with a greater number of cells and homogenize them in the same 300 ul MDA lysis buffer. They didn't think deproteinization would help. But you may want to use n-butanol to ex...

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Yes, that is correct: 0, 2, 4, 6, 8, 10 nmol per well, since you have used half the suggested standard volume. Reporting nmol/mg should be fine, but converting to ng/mg is easy enough to do. I am not sure why the other kit we discussed uses uM units bu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"