製品の概要

  • 製品名Anti-Lipid A antibody
    Lipid A 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Lipid A
  • 特異性The antibody was tested in direct IF on cells fixed with acetone and the secondary antibody was labeled with FITC.
  • アプリケーション適用あり: Conjugation, ICC/IFmore details
  • 種交差性
    Reactive with numerous members of the Enterobacteriaciae. Pseudomonas aeruginosa, Klebsiella pneumoniae, E. coli O157, Salmonella enteriditis, Enterobacter aerogenes, E. hermanii, Yersinia enterocolitica and Shigella sonnei. Not yet tested in other species.
  • 免疫原

    Whole cells prep of Lipid A from E. coli O157.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)Sodium sulfate precipitation and ion-exchange chromatography.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab20001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Conjugation Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution. Fix with acetone.

ターゲット情報

  • 関連性Lipid A is a lipid component of an endotoxin held responsible for toxicity of Gram-negative bacteria. Sensing of lipid A by the human immune system may also be critical for the onset of immune responses to Gram-negative infection, and for the subsequent successful fight against the infection. Lipid A is located at one end of the lipopolysaccharide (LPS, also called endotoxin) molecule, and anchors the LPS to the outer membrane of a Gram-negative bacteria. Many of the immune activating abilities of LPS can be contributed to the lipid A unit. It is a very potent stimulant of the immune system, activating cells (for example, monocytes or macrophages) at picogram per milliliter quantities. When present in the body at high concentrations during a Gram-negative bacterial infection, it may cause shock and death by an "out of control" excessive immune reaction.
  • 細胞内局在Secreted

Anti-Lipid A antibody (ab20001) 使用論文

This product has been referenced in:
  • Miyata ST  et al. Vibrio cholerae requires the type VI secretion system virulence factor VasX to kill Dictyostelium discoideum. Infect Immun 79:2941-9 (2011). Read more (PubMed: 21555399) »

See 1 Publication for this product

Product Wall

Application ELISA
Sample Mouse Serum (also plasma)
Specification also plasma
Type Other
Blocking step Pierce protein free buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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投稿 Jan 14 2014

Abreviews
Application Western blot
Sample Acinetobacter baumannii Cell lysate - whole cell (bacteria)
Loading amount 300000 cells
Specification bacteria
Gel Running Conditions Non-reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 minute(s) · Concentration: 0.3% · Temperature: 20°C
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投稿 Jan 09 2012

Abreviews
Application Western blot
Sample Pseudomonas aeruginosa Cell lysate - whole cell (bacteria)
Loading amount 300000 cells
Specification bacteria
Gel Running Conditions Non-reduced Denaturing (gel 12%)
Blocking step Milk as blocking agent for 1 minute(s) · Concentration: 0.3% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jan 05 2012

Code de remise : XXX%XXX-XXXX Date d’expiration : JJ MM AAAA Je suis ravi que vous souhaitiez participer à notre offre et tester ab20001 chez Acinetobacter baumannii. Le code de réduction correspondant est 100%ABR-E7TFB. Ce code de réduction ...

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Thank you for contacting us. Lab let me know that an affinity constant of Catalog ab20001, Goat anti Lipid A, has not been determined. This antibody recognizes the lipid A portion of LPS . When tested by IFA, the antibody shows strongest reac...

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Thank you for your enquiry. The antibody was tested in direct IF on cells fixed with acetone and the secondary antibody was labeled with FITC. I have added this information to the online datasheet. I hope this information helps, please do not he...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"