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Our Abpromise guarantee covers the use of ab8467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Agglutination||Use at an assay dependent concentration. Use NEAT.|
in phosphate buffered saline containing 4% BSA and 0.05% Tween.For ELISA prepare bacteria suspension in PBS (5x10^8 bact./ml). Coat ELISA plate with 100 µl/well for 1 h. at 37°C, store overnight at 4°C and wash 5x with tapwater with 0.05% Tween (TT). Incubate with diluted antibody, 1 h. at 37°C. Wash 5x with TT. Incubate with appropriate conjugate (anti-mouse Ig enzyme labeled antibody). Add substrate solution, incubate, stop reaction and read optical density.
|WB||Use at an assay dependent concentration. PubMed: 21873161|