The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/16000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 57 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin.
High level of expression in the liver, placenta, lung, thyroid, kidney, testis and in the corpus luteum of the ovary. Expressed also in coronary artery endothelial cells, umbilical vein endothelial cells and in hepatocyte and osteosarcoma cell lines. Not detected in heart, brain and muscle.
Belongs to the AB hydrolase superfamily. Lipase family. Contains 1 PLAT domain.
The observed molecular weight of approximately 70 kDa corresponds to earlier findings in literature with different antibodies (Gauster et al , J Clin Endocrinol Metab. 2007 Jun;92(6):2256-63. Epub 2007 Mar 13.;
Both bands were successfully blocked by incubation with the immunising peptide.