製品の概要

  • 製品名Anti-LIPG antibody
    LIPG 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LIPG
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Cow, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human LIPG.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human kidney tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab100987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250. Detects a band of approximately 62 kDa (predicted molecular weight: 57 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin.
  • 組織特異性High level of expression in the liver, placenta, lung, thyroid, kidney, testis and in the corpus luteum of the ovary. Expressed also in coronary artery endothelial cells, umbilical vein endothelial cells and in hepatocyte and osteosarcoma cell lines. Not detected in heart, brain and muscle.
  • 配列類似性Belongs to the AB hydrolase superfamily. Lipase family.
    Contains 1 PLAT domain.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EDL antibody
    • EL antibody
    • Endothelial cell-derived lipase antibody
    • Endothelial lipase antibody
    • Endothelial lipase precursor antibody
    • Lipase endothelial antibody
    • LIPE_HUMAN antibody
    • LIPG antibody
    • Lipoprotein lipase H antibody
    • PRO719 antibody
    see all

Anti-LIPG antibody 画像

  • Anti-LIPG antibody (ab100987) at 1/250 dilution + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • ICC/IF image of ab100987 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab100987, µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa cells at 5µg/ml.

Anti-LIPG antibody (ab100987) 使用論文

ab100987 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton X100 in PBS
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

投稿 Sep 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"