Lipase Assay Kit II (Colorimetric) (ab102525)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
製品の概要
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製品名
Lipase Assay Kit II (Colorimetric)
Lipase キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
アッセイタイプ
Enzyme activity -
全工程の試験時間
1h 30m -
製品の概要
Lipase Assay Kit II (Colorimetric) (ab102525) provides a simple, sensitive, and reliable assay for rapid analysis of Lipase activity.
In the lipase assay, Lipases hydrolyze a specific substrate to generate a product which reacts with the DTNB probe to generate color (Absorbance=412 nm). This kit is suitable for high throughput analysis (HTP).
Lipase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader at 3 min and at between 60 and 90 min -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K723 Lipase Activity Colorimetric Assay Kit II. K723-100 is the same size as the 100 test size of ab102525.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Assay Buffer V 1 x 25ml DTNB 1 vial Lipase Positive Control 1 vial Lipase Substrate II 1 x 500µl TNB Standard 1 vial -
研究分野
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関連性
Lipases perform essential roles in the digestion, transport and processing of dietary lipids (e.g. fats and oils) in living organisms. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglyceride to monoglyceride and free fatty acid. Pancreatic lipase monitoring is also used to help diagnose Crohn's disease, cystic fibrosis and celiac disease. Damage to the pancreas can exhibit a 5-10 fold increase of serum lipase levels within 24 to 48 hours.
関連製品
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab102525 は 1 報の論文で使用されています。
- Latorre J et al. Specific adipose tissue Lbp gene knockdown prevents diet-induced body weight gain, impacting fat accretion-related gene and protein expression. Mol Ther Nucleic Acids 27:870-879 (2022). PubMed: 35141047