製品の概要

  • 製品名Lipase Activity Assay Kit (Colorimetric)
    Lipase キット 製品一覧
  • テスト
    100 x 1 assay
  • サンプルの種類
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
  • アッセイタイプEnzyme activity
  • 検出感度
    0.02 mU/well
  • 全工程の試験時間
    1h 30m
  • 製品の概要

    Lipase Activity Assay Kit (Colorimetric) (ab102524) is a product where lipase hydrolyzes a triglyceride substrate to form glycerol which is quantified enzymatically by via monitoring a linked change in the OxiRed probe absorbance (OD=570nm).
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    This assay is rapid, simple, sensitive, and reliable for activity screening of lipase. This kit detects lipase activity as low as 0.02mU per well.

    For HTP analysis, we recommend Lipase Detection Kit II (Colorimetric) (ab102525) or Lipase Detection Kit III (Fluorometric) (ab118969).

  • アプリケーション適用あり: Functional Studiesmore details

法規制情報

製品の特性

  • 関連性Lipases perform essential roles in the digestion, transport and processing of dietary lipids (e.g. fats and oils) in living organisms. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglyceride to monoglyceride and free fatty acid. Pancreatic lipase monitoring is also used to help diagnose Crohn's disease, cystic fibrosis and celiac disease. Damage to the pancreas can exhibit a 5-10 fold increase of serum lipase levels within 24 to 48 hours.

アプリケーション

Our Abpromise guarantee covers the use of ab102524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

Lipase Activity Assay Kit (Colorimetric) 画像

  • Sample Timeline
  • Glycerol standard Curve

プロトコール

Lipase Activity Assay Kit (Colorimetric) (ab102524) 使用論文

This product has been referenced in:
  • Mondragon A  et al. Divergent effects of liraglutide, exendin-4, and sitagliptin on beta-cell mass and indicators of pancreatitis in a mouse model of hyperglycaemia. PLoS One 9:e104873 (2014). Read more (PubMed: 25119717) »

See 1 Publication for this product

Product Wall


Since the lab has not tried vegetable oil themselves, they do not have any recommendations/ comments on what the most optimal protocol for such use would be. That would have to be optimized by the end user.

Thank you for your inquiry.

I heard back from the lab that the efficiency of the assay depends on how well the serum was frozen, how many times it has been freeze thawed since then etc.

If the serum is of good quality, the lab sugge...

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the Assay Buffer included with the kit can be used to open cells, thus producing a homogenate that can be assed for lipase activity.

It is recommended to use a dounce homogenizer to homogenize cells or tissues here, although your biomasher ma...

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Thank you for contacting Abcam.

The substrate for ab102524 is also a triglyceride derivative. The datasheet product overview states, "In Abcam's Lipase Assay Kit (Colorimetric), lipase hydrolyzes a triglyceride substrate to form glycerol ...

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Thank you for your patience.

I am sorry this kit component did not conatin the amount as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx. T...

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Thank you for contacting us and for letting us know about this issue.

I am in touch with the supplying lab who has agreed to provide another vial of the glycerol standard as well as with our internal department to arrange for this.

...

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Thank you for your email.

I have received the following email form lab;

For the standards, you have to take the OD only at the final time point. The OD values of the various concentrations of glycerol will increase with time and final...

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Thank you for sending the data.

Unfortunately the graph only, is not enough for the data analysis. We need to check all the protocol details e.g. species, type of samples used, sample dilutions and the raw data etc.

I just had chat w...

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Thank you for letting me know.

I don't know what could have happened there but if you have any similar problems in the future please do let us know.

May I ask, when you called this morning, did you speak to any of our Scientific Sup...

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Thank you for contacting us and sorry for the delay in getting back to you.

I am sorry for the confusion caused by how the protocol has been worded for the Lipase Detection Kit (Colorimetric), ab102524. I can confirm that as the protocol stat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"