製品の概要

  • 製品名
    Lipase Activity Assay Kit (Colorimetric)
    Lipase キット 製品一覧
  • サンプルの種類
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
  • アッセイタイプ
    Enzyme activity
  • 検出感度
    0.02 mU/well
  • 全工程の試験時間
    1h 30m
  • 製品の概要

    Lipase Activity Assay Kit (Colorimetric) (ab102524) is a product where lipase hydrolyzes a triglyceride substrate to form glycerol which is quantified enzymatically by via monitoring a linked change in the OxiRed probe absorbance (OD=570nm).
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    This assay is rapid, simple, sensitive, and reliable for activity screening of lipase. This kit detects lipase activity as low as 0.02mU per well.

    For HTP analysis, we recommend Lipase Activity Assay Kit II (Colorimetric) (ab102525) or Lipase Activity Assay Kit III (Fluorometric) (ab118969).

  • アプリケーション
    適用あり: Functional Studiesmore details

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab102524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

画像

  • Sample Timeline
  • Glycerol standard Curve

プロトコール

参考文献

This product has been referenced in:
  • Sakamuri SSVP  et al. Absence of Tissue Inhibitor of Metalloproteinase-4 (TIMP4) ameliorates high fat diet-induced obesity in mice due to defective lipid absorption. Sci Rep 7:6210 (2017). Read more (PubMed: 28740132) »
  • Mondragon A  et al. Divergent effects of liraglutide, exendin-4, and sitagliptin on beta-cell mass and indicators of pancreatitis in a mouse model of hyperglycaemia. PLoS One 9:e104873 (2014). Read more (PubMed: 25119717) »

See all 2 Publications for this product

レビューと Q&A


Since the lab has not tried vegetable oil themselves, they do not have any recommendations/ comments on what the most optimal protocol for such use would be. That would have to be optimized by the end user.

Thank you for your inquiry.

I heard back from the lab that the efficiency of the assay depends on how well the serum was frozen, how many times it has been freeze thawed since then etc.

If the serum is of good quality, the lab sugge...

Read More

the Assay Buffer included with the kit can be used to open cells, thus producing a homogenate that can be assed for lipase activity.

It is recommended to use a dounce homogenizer to homogenize cells or tissues here, although your biomasher ma...

Read More

Thank you for contacting Abcam.

The substrate for ab102524 is also a triglyceride derivative. The datasheet product overview states, "In Abcam's Lipase Assay Kit (Colorimetric), lipase hydrolyzes a triglyceride substrate to form glycerol ...

Read More

Thank you for your patience.

I am sorry this kit component did not conatin the amount as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx. T...

Read More

Thank you for contacting us and for letting us know about this issue.

I am in touch with the supplying lab who has agreed to provide another vial of the glycerol standard as well as with our internal department to arrange for this.

...

Read More

Thank you for your email.

I have received the following email form lab;

For the standards, you have to take the OD only at the final time point. The OD values of the various concentrations of glycerol will increase with time and final...

Read More

Thank you for sending the data.

Unfortunately the graph only, is not enough for the data analysis. We need to check all the protocol details e.g. species, type of samples used, sample dilutions and the raw data etc.

I just had chat w...

Read More

Thank you for letting me know.

I don't know what could have happened there but if you have any similar problems in the future please do let us know.

May I ask, when you called this morning, did you speak to any of our Scientific Sup...

Read More

Thank you for contacting us and sorry for the delay in getting back to you.

I am sorry for the confusion caused by how the protocol has been worded for the Lipase Detection Kit (Colorimetric), ab102524. I can confirm that as the protocol stat...

Read More

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録