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Synthetic peptide conjugated to KLH from a region corresponding to the N-terminal of Human Lin28B.
Our Abpromise guarantee covers the use of ab115698 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50 - 1/100. Predicted molecular weight: 27 kDa.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing HL-60 cells stained with ab115698 (green line). The cells were fixed with paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 3% BSA to block non-specific protein-protein interactions followed by the antibody (ab115698, 1µg/1x106 cells) for 60 min at 37ºC. The secondary antibody used was FITC conjugated goat anti-rabbit IgG at 1/200 dilution for 40 min at room temperature. Blank control (blue line) was used under the same conditions. Acquisition of >10,000 events was performed.
ab115698 has not yet been referenced specifically in any publications.