製品の概要

  • 製品名Anti-Lin28 antibody [PR-4C9]
    Lin28 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [PR-4C9] to Lin28
  • アプリケーション適用あり: WB, Dot blotmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 100-150 (SAKGLESIRV TGPGGVFCIG SERRPKGKSM QKRRSKGDRC YNCGGLDHHA K) of Human Lin28 conjugated to Ovalbumin (NP_078950).

  • ポジティブ・コントロール
    • CHO-Lin28 transfectant, Mouse F9, 2102, NTERA-2 and HEK293-Lin28 transfectant cell lysates.
  • 特記事項This product is for Research Use Only and not to be used for any commercial resale purpose, including, but not limited to, using the product as part of any developed assay technology such as ELISA, ELISPOT, or Multiplex assay that is sold to another end-user.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab95852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 23 kDa.
Dot blot 1/500.

ターゲット情報

  • 機能Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
  • 組織特異性Expressed in embryonic stem cells (ES cells), placenta and testis.
  • 配列類似性Belongs to the lin-28 family.
    Contains 2 CCHC-type zinc fingers.
    Contains 1 CSD (cold-shock) domain.
  • 発生段階Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.
  • ドメインThe CSD domain is required for function in muscle differentiation.
  • 細胞内局在Cytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AL024421 antibody
    • CSDD1 antibody
    • FLJ12457 antibody
    • Lin 28 antibody
    • Lin 28 homolog (C. elegans) antibody
    • Lin 28 homolog A (C. elegans) antibody
    • Lin 28 homolog A antibody
    • Lin 28 homolog antibody
    • Lin-28A antibody
    • Lin28 antibody
    • Lin28, C. elegans, homolog of, A antibody
    • LIN28A antibody
    • LN28A_HUMAN antibody
    • Protein lin-28 homolog A antibody
    • RNA binding protein lin 28 antibody
    • Tex17 antibody
    • ZCCHC1 antibody
    • Zinc finger CCHC domain containing 1 antibody
    • Zinc finger CCHC domain containing protein 1 antibody
    • Zinc finger CCHC domain-containing protein 1 antibody
    see all

Anti-Lin28 antibody [PR-4C9] 画像

  • All lanes : Anti-Lin28 antibody [PR-4C9] (ab95852) at 1 µg/ml

    Lane 1 : CHO-Lin28 transfectant cell lysate at 20 µg
    Lane 2 : Untransfected CHO cell lysate at 20 µg
    Lane 3 : Mouse F9 cell lysate at 10 µg
    Lane 4 : 2102 cell lysat at 20 µg
    Lane 5 : NTERA-2 cell lysate at 20 µg


    Predicted band size : 23 kDa
  • Dot Blot showing specific detection of Lin28 in transfected HEK293 cell lysates (non-denatured) using ab95852 at 1/500 dilution.

Anti-Lin28 antibody [PR-4C9] (ab95852) 使用論文

ab95852 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"