Synthetic peptide derived from Human Lin28 (within 50 amino acids of N-terminus) conjugated to Ovalbumin (NP_078950).
HEK-293-Lin28 transfectant, NTERA-2, and Mouse F9 cell lysates.
特記事項This product is for Research Use Only and not to be used for any commercial resale purpose, including, but not limited to, using the product as part of any developed assay technology such as ELISA, ELISPOT, or Multiplex assay that is sold to another end-user.
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500. Predicted molecular weight: 23 kDa.
機能Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
組織特異性Expressed in embryonic stem cells (ES cells), placenta and testis.
配列類似性Belongs to the lin-28 family. Contains 2 CCHC-type zinc fingers. Contains 1 CSD (cold-shock) domain.
発生段階Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.
ドメインThe CSD domain is required for function in muscle differentiation.
細胞内局在Cytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
Zinc finger CCHC domain containing protein 1 antibody
Zinc finger CCHC domain-containing protein 1 antibody
Anti-Lin28 antibody [PR-3G10] 画像
Western blot - Lin28 antibody [PR-3G10] (ab95855)
All lanes : Anti-Lin28 antibody [PR-3G10] (ab95855) at 2 µg/ml
Lane 1 : Untransfected HEK293 cell lysate at 4 µg Lane 2 : HEK293-Lin28 transfectant cell lysate at 4 µg Lane 3 : NTERA-2 cell lysate at 20 µg Lane 4 : Mouse F9 cell lysate at 10 µg
Predicted band size : 23 kDa
Dot Blot - Lin28 antibody [PR-3G10] (ab95855)
Dot Blot showing specific detection of Lin28 in transfected HEK293 cell lysates (non-denatured) using ab95855 at 1/500 dilution.
293-Nanog lysate (x3)
293-Oct4 lysate (x3)
293-SOX2 lysate (x3)
293-Lin28 lysate (x3)
Anti-Lin28 antibody [PR-3G10] (ab95855) 使用論文
has not yet been referenced specifically in any publications.