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Recombinant tagged fragment, corresponding to amino acids 93-209 of Human Lin28 expressed in E. coli strain BL21 (DE3)
Our Abpromise guarantee covers the use of ab76369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Lin28 (ab63740). For sandwich ELISA, use this antibody as Capture at 0.2 µg/ml with Rabbit polyclonal to Lin28 (ab63740) as Detection.|
|WB||1/500 - 1/2000. Predicted molecular weight: 23 kDa.|
|ICC/IF||1/200 - 1/1000.|
|Flow Cyt||Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
ab76369 at 1/2000 dillution staining Lin28 in NTERA-2 cells by Immunocytochemistry/ Immunofluorescence. The green color in image show positive staining with primary antibody while nuclei were stained blue with DRAQ5 fluorescent DNA dye.
ab76369 has not yet been referenced specifically in any publications.