製品の概要

  • 製品名Anti-LIMS1 antibody [EP1944]
    LIMS1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1944] to LIMS1
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human LIMS1.

  • ポジティブ・コントロール
    • 293T, HeLa, Jurkat, Human fetal kidney, and Human spleen cell lysates. Human kidney tissue. HeLa cells.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Effector of integrin and growth factor signaling, coupling surface receptors to downstream signaling molecules involved in the regulation of cell survival, cell proliferation and cell differentiation. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway.
    • 組織特異性Expressed in most tissues except in the brain.
    • 配列類似性Contains 5 LIM zinc-binding domains.
    • 細胞内局在Cell junction > focal adhesion. Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
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    Anti-LIMS1 antibody [EP1944] 画像

    • All lanes : Anti-LIMS1 antibody [EP1944] (ab108609) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : Human fetal kidney lysate
      Lane 5 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 37 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical staining of paraffin-embedded Human kidney tissue using ab108609 at 1/100 dilution.
    • Immunofluorescent staining of HeLa cells using ab108609 at 1/100 dilution.

    Anti-LIMS1 antibody [EP1944] (ab108609) 使用論文

    This product has been referenced in:
    • Li A  et al. The interactome of LIM domain proteins: the contributions of LIM domain proteins to heart failure and heart development. Proteomics 12:203-25 (2012). Read more (PubMed: 22253135) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab108609.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"