製品の概要

  • 製品名Anti-LIMS1 antibody [EP1943Y]
    LIMS1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1943Y] to LIMS1
  • アプリケーション適用あり: WB, Flow Cytmore details
    適用なし: ICC,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues within the LIM zinc-binding 3 region of human LIMS1

  • ポジティブ・コントロール
    • Jurkat, K562, HeLa and 293 cell lysates.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Detects a band of approximately 34-37 kDa (predicted molecular weight: 37 kDa).
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for ICC,IHC-P or IP.
  • ターゲット情報

    • 機能Effector of integrin and growth factor signaling, coupling surface receptors to downstream signaling molecules involved in the regulation of cell survival, cell proliferation and cell differentiation. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway.
    • 組織特異性Expressed in most tissues except in the brain.
    • 配列類似性Contains 5 LIM zinc-binding domains.
    • 細胞内局在Cell junction > focal adhesion. Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
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      • Lims1 antibody
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      • PINCH antibody
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    Anti-LIMS1 antibody [EP1943Y] 画像

    • All lanes : Anti-LIMS1 antibody [EP1943Y] (ab76112) at 1/5000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 37 kDa
      Observed band size : 34-37 kDa (why is the actual band size different from the predicted?)

    Anti-LIMS1 antibody [EP1943Y] (ab76112) 使用論文

    ab76112 has not yet been referenced specifically in any publications.

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