製品の概要

  • 製品名Anti-LIMS1 antibody
    LIMS1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LIMS1
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Chicken, Cow, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human LIMS1.

    (Peptide available as ab88119.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysate: A549

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab78814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Effector of integrin and growth factor signaling, coupling surface receptors to downstream signaling molecules involved in the regulation of cell survival, cell proliferation and cell differentiation. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway.
  • 組織特異性Expressed in most tissues except in the brain.
  • 配列類似性Contains 5 LIM zinc-binding domains.
  • 細胞内局在Cell junction > focal adhesion. Cell membrane.
  • Information by UniProt
  • 参照データベース
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Anti-LIMS1 antibody 画像

  • Anti-LIMS1 antibody (ab78814) at 1 µg/ml + A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 37 kDa


    Exposure time : 15 minutes
  • ICC/IF image of ab78814 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78814, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-LIMS1 antibody (ab78814) 使用論文

ab78814 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab78814.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"