製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to LIM1
  • 特異性
    Does not appear to crossreact with LIM5 (the closest homologue of LIM1 in frog) on tissue sections but does crossreact with LIM5 in Western blot and immunoprecipitation.
  • アプリケーション
    適用あり: IHC-P, ICC, WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Xenopus laevis, Fish
  • 免疫原

    C-terminal portion of frog LIM-1 protein.

  • ポジティブ・コントロール
    • Tested with P19 cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/1000.
ICC 1/500.
WB 1/3000 - 1/6000. Predicted molecular weight: 44 kDa.
IP 1/200.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Potential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis.
  • 組織特異性
    Expressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58% of acute myeloid leukemia (AML) cell lines.
  • 配列類似性
    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • ドメイン
    The LIM domains exert a negative regulatory function and disruption of the LIM domains produces an activated form. In addition, two activation domains and a negative regulatory domain exist C-terminally to the homeobox.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hLim-1 antibody
    • Homeo box protein Lim 1 antibody
    • Homeo box protein Lim1 antibody
    • Homeobox protein Lim 1 antibody
    • Homeobox protein Lim-1 antibody
    • Homeobox protein Lim1 antibody
    • LHX 1 antibody
    • LHX1 antibody
    • LHX1_HUMAN antibody
    • LIM 1 antibody
    • LIM homeo box 1 antibody
    • LIM homeo box protein 1 antibody
    • LIM homeobox 1 antibody
    • LIM homeobox protein 1 antibody
    • LIM-1 antibody
    • LIM/homeobox protein Lhx 1 antibody
    • LIM/homeobox protein Lhx1 antibody
    • MGC126723 antibody
    • MGC138141 antibody
    see all

画像

  • ab14554 staining LIM1 in human fetal kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.5% Triton X-100 in PBS and blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 37°C. An Alexa Fluor® 488-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Actin filaments stained with phalloidin (red).

    See Abreview

  • ab14554 at 1/200 dilution staining mouse postnatal day 5 retina cells (known to express LIM 1) by ICC/IF. The cells were formaldehyde fixed and incubated with the antibody for 72 hours. An Alex-Fluor 488 conjugated donkey anti-rabbit antibody was used as the secondary. The image shows strong horizontal cell process labeling with fainter nuclear label.

    See Abreview

参考文献

This product has been referenced in:
  • Lui NC  et al. Lhx1/5 control dendritogenesis and spine morphogenesis of Purkinje cells via regulation of Espin. Nat Commun 8:15079 (2017). Read more (PubMed: 28516904) »
  • Liu J  et al. Chromatin landscape defined by repressive histone methylation during oligodendrocyte differentiation. J Neurosci 35:352-65 (2015). ICC/IF . Read more (PubMed: 25568127) »

See all 5 Publications for this product

レビューと Q&A

Sure, as the antibody is not working as expected, I would be happy to send a replacement or offer a credit or refund. What would you prefer?

Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Tissue sections (nephric duct (chiken))
Permeabilization
Yes - 0.25% triton in PBS
Specification
nephric duct (chiken)
Blocking step
(agent) for 15 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
Paraformaldehyde
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投稿 Nov 24 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Human Cell (fetal kidney cells)
Specification
fetal kidney cells
Permeabilization
Yes - 0.5% triton in pbs
Fixative
Formaldehyde
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投稿 Oct 24 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Chicken Tissue sections (E5 Spinal cord)
Specification
E5 Spinal cord
Permeabilization
Yes - triton x-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 27 2013

Thank you for your reply. I am sending a vial of ab82844 free of charge on the order ***. This is in stock and I expect it to arrive tomorrow. Please keep me updated on the results with this replacement antibody, and let me know if you h...

Read More

Thanks for your reply and I look forward to receiving your image. Regarding the 150 kDa band, I have looked through our records and briefly in the literature and have found no evidence that LIM1 should run that high, so this band is most likely ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (postnatal day 5 retina)
Specification
postnatal day 5 retina
Fixative
Formaldehyde
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Oct 17 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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