製品の概要

  • 製品名Anti-LIM kinase 2 (phospho T505) antibody
    LIM kinase 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LIM kinase 2 (phospho T505)
  • 特異性This antibody is specific for LIM kinase 2 only when phosphorylated at threonine 505.
  • アプリケーション適用あり: WB, IHC-P, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from Human LIM kinase 2 around the phosphorylation site of threonine 505.

  • ポジティブ・コントロール
    • Breast carcinoma tissue and MCF7 cell extract.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab38499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 72 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
ICC/IF Use a concentration of 1 - 5 µg/ml.

ターゲット情報

  • 機能Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
  • 組織特異性Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
  • 配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylated on serine and/or threonine residues by ROCK1.
  • 細胞内局在Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • LIM domain kinase 2 antibody
    • LIMK 2 antibody
    • LIMK-2 antibody
    • Limk2 antibody
    • LIMK2_HUMAN antibody
    see all

Anti-LIM kinase 2 (phospho T505) antibody 画像

  • ab38499 at a 1:50 dilution staining LIM kinase 2 in Human breast carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.

    Left image : untreated.

    Right image : treated with phosphopeptide.

  • All lanes : Anti-LIM kinase 2 (phospho T505) antibody (ab38499) at 1/500 dilution

    Lane 1 : MCF7 cell extract (5-30ug of total protein) pre-incubated with phosphopeptide.
    Lane 2 : MCF7 cell extract (5-30ug of total protein).

    Secondary
    Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L)

    Predicted band size : 72 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab38499 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38499, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-LIM kinase 2 (phospho T505) antibody (ab38499) 使用論文

This product has been referenced in:
  • Gardinier J  et al. P2Y2 receptors regulate osteoblast mechanosensitivity during fluid flow. Am J Physiol Cell Physiol 306:C1058-67 (2014). Read more (PubMed: 24696143) »
  • Ray S  et al. LIM kinase regulation of cytoskeletal dynamics is required for salivary gland branching morphogenesis. Mol Biol Cell 25:2393-407 (2014). Read more (PubMed: 24966172) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (LNCaP (prostate cancer cell line))
Loading amount 10 µg
Specification LNCaP (prostate cancer cell line)
Gel Running Conditions Reduced Denaturing (7% tris-acetate gel)
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
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投稿 Jun 24 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"