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Synthetic peptide corresponding to residues near the C-terminus of human LIM kinase 2.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab45165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||1/50 - 1/100.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/2000. Predicted molecular weight: 72 kDa.|
Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
Lane 2: LIMK2 (LIM kinase 2) knockout HAP1 whole cell lysate (40 µg)
Lane 3: Jurkat whole cell lysate (40 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab45165 observed at 72 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab45165 was shown to specifically recognize LIMK2 (LIM kinase 2) in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed with knockout cells were examined. Wild-type and LIMK2 (LIM kinase 2) knockout samples were subjected to SDS-PAGE. Ab45165 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
ab45165 staining human LIM kinase 2 in human ovary carcinoma by immunohistochemistry using paraffin embedded tissue.