Anti-LIM Kinase 1 (phospho T508) 抗体 (ab38508)

製品の概要

  • 製品名
    Anti-LIM Kinase 1 (phospho T508) antibody
    LIM Kinase 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LIM Kinase 1 (phospho T508)
  • 特異性
    This antibody is specific for LIM Kinase only when phosphorylated at threonine 508. It recognizes both LIMK1 and LIMK2 from human, mouse, and rat.
  • アプリケーション
    適用あり: WB, IHC-P, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from Human LIM Kinase around the phosphorylation site of threonine 508.

  • ポジティブ・コントロール
    • IHC-P: Human breast carcinoma tissue slides. WB: COLO and MCF7 cell extracts.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab38508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 73 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/10000.
ICC/IF 1/100. PubMed: 19208621

ターゲット情報

  • 機能
    Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
  • 組織特異性
    Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
  • 関連疾患
    Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • 配列類似性
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Autophosphorylated.
    Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
    Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
  • 細胞内局在
    Cytoplasm. Cell projection > growth cone.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC 2.7.1.37 antibody
    • LIM domain containing protein kinase antibody
    • LIM domain kinase 1 antibody
    • LIM kinase antibody
    • LIM motif containing protein kinase antibody
    • LIMK 1 antibody
    • LIMK antibody
    • LIMK-1 antibody
    • limk1 antibody
    • LIMK1_HUMAN antibody
    see all

Anti-LIM Kinase 1 (phospho T508) antibody 画像

  • ab38508 at a 1:50 dilution staining LIM Kinase in Human breast carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.

    Left image : untreated.

    Right image : treated with phosphopeptide.

  • All lanes : Anti-LIM Kinase 1 (phospho T508) antibody (ab38508) at 1/500 dilution

    Lane 1 : Extract of COLO cells + peptide.
    Lane 2 : Extract of COLO cells.
    Lane 3 : Extract of MCF7 cells.

    Lysates/proteins at 30 µg per lane.

    Secondary
    Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)

    Predicted band size : 73 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Lanes can be loaded with 5-30µg of total protein. The arrow next to the image points to LIM Kinase (phospho T508).
  • ab38508 staining LIM Kinase 1 in mouse brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    bdKO mice were anesthetized and transcardially perfused with PBS, followed by 4% paraformaldehyde (PFA) prior to brain dissection. Brains were then postfixed in 4% PFA for 2 days, embedded in paraffin and sectioned.

Anti-LIM Kinase 1 (phospho T508) antibody (ab38508) 使用論文

This product has been referenced in:
  • Tsai CS  et al. Thrombomodulin regulates monocye differentiation via PKCd and ERK1/2 pathway in vitro and in atherosclerotic artery. Sci Rep 6:38421 (2016). WB ; Human . Read more (PubMed: 27910925) »
  • Ray S  et al. LIM kinase regulation of cytoskeletal dynamics is required for salivary gland branching morphogenesis. Mol Biol Cell 25:2393-407 (2014). Read more (PubMed: 24966172) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Brain)
Loading amount
20 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 27 2011

Thank you for your patience. I have been in contact with the originator of this antibody, and below is the detailed antigen retrieval protocol. The method of antigen retrieval was used for IHC-P as following: Deparaffinization 1. Incubate slide a...

Read More

Thank you for your enquiry. From data of recent testing, this antibody recognizes both LIMK1 and LIMK2 from human, mouse, and rat. I have clarified this on the datasheet; should you have further questions please don't hesitate to contact me,

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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