Anti-LIM Kinase 1 抗体 [EPR912] (ab108507)

製品の概要

  • 製品名Anti-LIM Kinase 1 antibody [EPR912]
    LIM Kinase 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR912] to LIM Kinase 1
  • アプリケーション適用あり: WBmore details
    適用なし: Flow Cyt,ICC,IHC-P or IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N terminal region of Human LIM Kinase 1.

  • ポジティブ・コントロール
    • U-87 MG, SH SY5Y and Ramos cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab108507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
  • 追加情報Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • ターゲット情報

    • 機能Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
    • 組織特異性Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
    • 関連疾患Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
    • 配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 2 LIM zinc-binding domains.
      Contains 1 PDZ (DHR) domain.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Autophosphorylated.
      Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
      Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
    • 細胞内局在Cytoplasm. Cell projection > growth cone.
    • Information by UniProt
    • 参照データベース
    • 別名
      • EC 2.7.1.37 antibody
      • LIM domain containing protein kinase antibody
      • LIM domain kinase 1 antibody
      • LIM motif containing protein kinase antibody
      • LIMK 1 antibody
      • LIMK antibody
      • LIMK-1 antibody
      • limk1 antibody
      • LIMK1_HUMAN antibody
      see all

    Anti-LIM Kinase 1 antibody [EPR912] 画像



    • Predicted band size : 73 kDa

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: LIMK1 knockout HAP1 cell lysate (40 µg)
      Lane 3: PC12 cell lysate (20 µg)
      Lane 4: NIH3T3 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab108507 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab108507 was shown to specifically react with LIM Kinase 1 when LIM Kinase 1 knockout samples were used. Wild-type and LIM Kinase 1 knockout samples were subjected to SDS-PAGE. Ab108507 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-LIM Kinase 1 antibody [EPR912] (ab108507) at 1/1000 dilution

      Lane 1 : U-87 MG cell lysate
      Lane 2 : SH SY5Y cell lysate
      Lane 3 : Ramos cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 73 kDa

    Anti-LIM Kinase 1 antibody [EPR912] (ab108507) 使用論文

    ab108507 has not yet been referenced specifically in any publications.

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