製品の概要

  • 製品名Anti-LIM Kinase 1 antibody
    LIM Kinase 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LIM Kinase 1
  • 特異性ab39641 detects endogenous levels of total LIM Kinase 1 protein.
  • アプリケーション適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide (non-phosphorylated) derived from human LIM Kinase 1 around the phosphorylation site of Threonine 508.

  • ポジティブ・コントロール
    • A2780 and NIH/3T3 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab39641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
ELISA
  • 追加情報ELISA: 1/4000.
    ICC/IF: 1/100 (PMID 19208621).
    WB: 1/500 - 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
    • 組織特異性Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
    • 関連疾患Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
    • 配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 2 LIM zinc-binding domains.
      Contains 1 PDZ (DHR) domain.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Autophosphorylated.
      Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
      Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
    • 細胞内局在Cytoplasm. Cell projection > growth cone.
    • Information by UniProt
    • 参照データベース
    • 別名
      • EC 2.7.1.37 antibody
      • LIM domain containing protein kinase antibody
      • LIM domain kinase 1 antibody
      • LIM motif containing protein kinase antibody
      • LIMK 1 antibody
      • LIMK antibody
      • LIMK-1 antibody
      • limk1 antibody
      • LIMK1_HUMAN antibody
      see all

    Anti-LIM Kinase 1 antibody 画像

    • All lanes : Anti-LIM Kinase 1 antibody (ab39641) at 1/500 dilution

      Lane 1 : NIH/3T3 cell extracts
      Lane 2 : A2780 cell extracts


      Predicted band size : 73 kDa
      Observed band size : 73 kDa
    • ab39641 staining LIM Kinase 1 in the growth cones of Human DRG neurons by Immunocytochemistry/ Immunofluorescence.
      Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature and permeabilized (0.1% Triton X-100 and 1× phosphate-buffered saline). After blocking, cells were incubated for double staining with primary antibodies, followed by incubation with the appropriate Cy3-secondary antibody for 30 minutes and with 4',6-diamidino-2-phenylindole. F-actin staining with Alexa 488-phalloidin.

      Scale bar = 10µm.

    Anti-LIM Kinase 1 antibody (ab39641) 使用論文

    This product has been referenced in:
    • Tondeleir D  et al. Cells lacking ß-actin are genetically reprogrammed and maintain conditional migratory capacity. Mol Cell Proteomics : (2012). Read more (PubMed: 22448045) »
    • Montani L  et al. Neuronal Nogo-A Modulates Growth Cone Motility via Rho-GTP/LIMK1/Cofilin in the Unlesioned Adult Nervous System. J Biol Chem 284:10793-807 (2009). WB, ICC/IF ; Rat . Read more (PubMed: 19208621) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your enquiry. Following a sequence alignment; http://www.ebi.ac.uk/cgi-bin/jobresults/clustalw/clustalw-20061214-15265852.aln? It appears that there is a degree of sequence similarity between the Drosophila and Human homologs....

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