製品の概要

  • 製品名Anti-LIM Kinase 1 antibody [3G3]
    LIM Kinase 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3G3] to LIM Kinase 1
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein of Human LIM Kinase 1 produced in HEK293T cells (NP_002305) .

  • ポジティブ・コントロール
    • HEK293T cells transfected with LIM Kinase 1, COS7 cells transiently transfected with LIM Kinase 1
  • 特記事項Concentration 0.5~1.0 mg/ml (Lot Dependent)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 73 kDa.
ICC/IF 1/100.

ターゲット情報

  • 機能Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
  • 組織特異性Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
  • 関連疾患Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • 配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated.
    Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
    Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
  • 細胞内局在Cytoplasm. Cell projection > growth cone.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC 2.7.1.37 antibody
    • LIM domain containing protein kinase antibody
    • LIM domain kinase 1 antibody
    • LIM motif containing protein kinase antibody
    • LIMK 1 antibody
    • LIMK antibody
    • LIMK-1 antibody
    • limk1 antibody
    • LIMK1_HUMAN antibody
    see all

Anti-LIM Kinase 1 antibody [3G3] 画像

  • All lanes : Anti-LIM Kinase 1 antibody [3G3] (ab119084) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate transfected the pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected the pCMV6-ENTRY LIM Kinase 1

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 73 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC218058)
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIM Kinase 1 using ab119084 at a dilution of 1/100.

Anti-LIM Kinase 1 antibody [3G3] (ab119084) 使用論文

ab119084 has not yet been referenced specifically in any publications.

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