製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to LIGHT
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 61-170 of Human LIGHT

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab57901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
  • 追加情報
    WB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus.
    • 組織特異性
      Predominantly expressed in the spleen but also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines.
    • 配列類似性
      Belongs to the tumor necrosis factor family.
    • 翻訳後修飾
      N-glycosylated.
      The soluble form of isoform 1 derives from the membrane form by proteolytic processing.
    • 細胞内局在
      Cytoplasm; Secreted and Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD 258 antibody
      • CD258 antibody
      • CD258 antigen antibody
      • Delta transmembrane LIGHT antibody
      • Herpes virus entry mediator ligand antibody
      • Herpesvirus entry mediator A antibody
      • Herpesvirus entry mediator ligand antibody
      • herpesvirus entry mediator-ligand antibody
      • HVEM L antibody
      • HVEM-L antibody
      • HVEML antibody
      • Ligand for herpesvirus entry mediator antibody
      • LIGHT antibody
      • LTg antibody
      • soluble form antibody
      • TNF14 antibody
      • TNF14_HUMAN antibody
      • TNFSF 14 antibody
      • Tnfsf14 antibody
      • TNFSF14 protein antibody
      • TR 2 antibody
      • TR2 antibody
      • Tumor necrosis factor (ligand) superfamily, member 14 antibody
      • Tumor necrosis factor ligand superfamily member 14 antibody
      • Tumor necrosis factor ligand superfamily, member 14 antibody
      • Tumor necrosis factor receptor like 2 antibody
      • tumor necrosis factor receptor-like 2 antibody
      • Tumor necrosis factor superfamily member 14 antibody
      • Tumor necrosis factor superfamily member LIGHT antibody
      see all

    画像

    • LIGHT antibody (ab57901) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human spleen.
    • Western blot against tagged recombinant protein immunogen using ab57901 LIGHT antibody at 1ug/ml. Predicted band size of immunogen is 35 kDa

    参考文献

    This product has been referenced in:
    • Maker AV  et al. Genetic evidence that intratumoral T-cell proliferation and activation are associated with recurrence and survival in patients with resected colorectal liver metastases. Cancer Immunol Res 3:380-8 (2015). Read more (PubMed: 25600439) »

    See 1 Publication for this product

    レビューと Q&A

    Thank you very much for your call today and for letting us know about the trouble with ab57901.

    As we discussed, I'm sending a free of charge vial of ab90133 on the order ***, which should arrive late next week. Please keep me updated about t...

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    Thank you for contacting us and your interest in our products.

    I now have more information to share with you in regards to how the anti-LIGHT antibody (ab57901) was used in order to obtain the stainingpresented on the datasheet of this produc...

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    Thank you for contacting us.

    I am unfamiliar with the Ventana LIGHT product and was unable tro locate a reference to it on their website. We do however offer 5 antibodies against LIGHT. Three are Rabbit IgG polyclonals, the other two are prod...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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