Anti-LIFR 抗体 (ab101228)

製品の概要

  • 製品名Anti-LIFR antibody
    LIFR 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LIFR
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Horse, Human, Pig, Chimpanzee, Macaque Monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Mouse LIFR.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Mouse Placenta tissue lysate and in mouse placenta FFPE tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab101228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).

ターゲット情報

  • 機能Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells.
  • 関連疾患Defects in LIFR are the cause of Stueve-Wiedemann syndrome (SWS) [MIM:601559]; also knowns as Schwartz-Jampel syndrome type 2 (SJS2). SWS is a severe autosomal recessive condition and belongs to the group of the bent-bone dysplasias. SWS is characterized by bowing of the lower limbs, with internal cortical thickening, wide metaphyses with abnormal trabecular pattern, and camptodactyly. Additional features include feeding and swallowing difficulties, as well as respiratory distress and hyperthermic episodes, which cause death in the first months of life. The rare survivors develop progressive scoliosis, spontaneous fractures, bowing of the lower limbs, with prominent joints and dysautonomia symptoms, including temperature instability, absent corneal and patellar reflexes, and smooth tongue.
    Note=A chromosomal aberration involving LIFR is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(5;8)(p13;q12) with PLAG1.
  • 配列類似性Belongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 6 fibronectin type-III domains.
  • ドメインThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD118 antibody
    • CD118 antigen antibody
    • FLJ98106 antibody
    • FLJ99923 antibody
    • Leukemia inhibitory factor receptor alpha antibody
    • Leukemia inhibitory factor receptor antibody
    • LIF R antibody
    • LIF receptor antibody
    • LIF-R antibody
    • Lifr antibody
    • LIFR_HUMAN antibody
    • SJS2 antibody
    • STWS antibody
    • SWS antibody
    see all

Anti-LIFR antibody 画像

  • Anti-LIFR antibody (ab101228) at 1 µg/ml + Mouse Placenta Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 122 kDa
    Observed band size : 122 kDa
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 90 seconds
  • IHC image of LIFR staining in mouse placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab101228, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-LIFR antibody (ab101228) 使用論文

ab101228 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon)
Antigen retrieval step Heat mediated
Permeabilization No
Specification Colon
Blocking step UV inhibitor - Ventana as blocking agent for 4 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative Formaldehyde
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投稿 Jul 05 2016

Ich verstehe Ihre Bedenken.

Es tut mir leid, dass ich Ihnen in diesem Fall nicht mehr helfen konnte.

Ich wünsche Ihnen alles Gute bei der Suche und viel Erfolg mit Ihrem Experiment.

Sorry for the delay in getting back to you.

I now have some further information to share with you in regards to the antibody ab89792. This antibody is a mouse monoclonal which has been raised against a recombinant fragment of the human LIFR p...

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