製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Lhx4
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 287-336 (GSFSMDGTGQ SYQDLRDGSP YGIPQSPSSI SSLPSHAPLL NGLDYTVDSN) of Human Lhx4 (NP_203129).

  • ポジティブ・コントロール
    • 721_B cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    May play a critical role in the development of respiratory control mechanisms and in the normal growth and maturation of the lung.
  • 関連疾患
    Defects in LHX4 are the cause of pituitary hormone deficiency combined type 4 (CPHD4) [MIM:262700]; also known as short stature pituitary and cerebellar defects and small sella turcica. The disorder is characterized by short stature, pituitary and cerebellar defects, and small transverse depression crossing the midline on the superior surface of the body of the sphenoid bone which houses the pituitary gland.
    Note=A chromosomal aberration involving LHX4 may be a cause of acute lymphoblastic leukemia. Translocation t(1;14)(q25;q32) with IGHG1.
  • 配列類似性
    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CPHD4 antibody
    • Gsh 4 antibody
    • Gsh4 antibody
    • Lhx4 antibody
    • LHX4_HUMAN antibody
    • LIM Homeobox 4 antibody
    • LIM Homeobox gene 4 antibody
    • LIM homeobox protein 4 antibody
    • LIM/homeobox protein Lhx4 antibody
    see all

画像

  • Anti-Lhx4 antibody (ab84519) at 1 µg/ml (5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 41 kDa

プロトコール

参考文献

ab84519 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse embryonic spinal cord (cryosections))
Permeabilization
Yes - 0.1%Triton X-100
Specification
Mouse embryonic spinal cord (cryosections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Dr. Frederic Clotman

Verified customer

投稿 Nov 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録