Anti-LHX3/LIM 抗体 [EPR3344(2)] (ab124697)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3344(2)] to LHX3/LIM
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-LHX3/LIM antibody [EPR3344(2)]
LHX3/LIM 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3344(2)] to LHX3/LIM -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human LHX3/LIM aa 300-400. The exact sequence is proprietary.
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ポジティブ・コントロール
- 293T and Jurkat whole cell lysate (ab7899), Human brain and fetal lung tissue lysates; permeabilized Jurkat cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 6.84 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3344(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124697の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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特記事項 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
ターゲット情報
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機能
Acts as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with Pit-1. -
関連疾患
Defects in LHX3 are the cause of pituitary hormone deficiency combined type 3 (CPHD3) [MIM:221750]; also known as combined pituitary hormone deficiency with rigid cervical spine or sensorineural deafness with pituitary dwarfism. CPHD is characterized by a complete deficit in all but one (adrenocorticotropin) anterior pituitary hormone and a rigid cervical spine leading to limited head rotation. -
配列類似性
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains. -
ドメイン
The LIM domain specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 8022 Human
- Entrez Gene: 16871 Mouse
- Entrez Gene: 170671 Rat
- Omim: 600577 Human
- SwissProt: Q9UBR4 Human
- SwissProt: P50481 Mouse
- Unigene: 148427 Human
- Unigene: 386765 Mouse
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別名
- CPHD 3 antibody
- CPHD3 antibody
- DKFZp762A2013 antibody
see all
画像
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All lanes : Anti-LHX3/LIM antibody [EPR3344(2)] (ab124697) at 1/1000 dilution
Lane 1 : 293T(Human embryonic kidney epithelial cell) cell lysate
Lane 2 : Human brain tissue lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Human fetal lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 43 kDa -
ab124697 at 1/10 dilution, staining LHX3/LIM in permeabilized Jurkat cells by intracellular flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab124697 は 2 報の論文で使用されています。
- Feng Y et al. HOXD8/DIAPH2-AS1 epigenetically regulates PAX3 and impairs HTR-8/SVneo cell function under hypoxia. Biosci Rep 39:N/A (2019). PubMed: 30626726
- Patani R et al. Retinoid-independent motor neurogenesis from human embryonic stem cells reveals a medial columnar ground state. Nat Commun 2:214 (2011). PubMed: 21364553