製品の概要

  • 製品名Anti-LHX3 antibody
    LHX3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LHX3
  • 特異性ab26025 recognises LHX3.
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide: EKRLKKDAGR QRWGQYFRNM KRSRGGSKSD KDSVQEGQDS DAEVSFPDEP, corresponding to an sequence within internal sequence amino acids 217-266 of Human LHX3

  • ポジティブ・コントロール
    • Jurkat cell lysate, human lung tissue, human heart tissue

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab26025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/312500.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Acts as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with Pit-1.
  • 関連疾患Defects in LHX3 are the cause of pituitary hormone deficiency combined type 3 (CPHD3) [MIM:221750]; also known as combined pituitary hormone deficiency with rigid cervical spine or sensorineural deafness with pituitary dwarfism. CPHD is characterized by a complete deficit in all but one (adrenocorticotropin) anterior pituitary hormone and a rigid cervical spine leading to limited head rotation.
  • 配列類似性Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • ドメインThe LIM domain specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CPHD 3 antibody
    • CPHD3 antibody
    • DKFZp762A2013 antibody
    • LHX 3 antibody
    • LHX3 antibody
    • LHX3_HUMAN antibody
    • LIM 3 antibody
    • LIM homeobox 3 antibody
    • LIM homeobox gene 3 antibody
    • LIM homeobox protein 3 antibody
    • LIM/homeobox protein Lhx3 antibody
    • LIM/homeodomain protein LHX3 antibody
    • Lim3 antibody
    • M2 LHX3 antibody
    • mLim-3 antibody
    • mLIM3 antibody
    • P LIM antibody
    see all

Anti-LHX3 antibody 画像

  • Anti-LHX3 antibody (ab26025) at 1.25 µg/ml

    Predicted band size : 44 kDa
  • ab26025 at a concentration of 6.0 µg/ml staining human LHX3 by Immunohistochemistry in paraffin embedded lung tissue. Cells with positive label (as indicated by the arrows) are alveclar cells. Magnification 400X.
  • ab26025 at a concentration of 6.0 µg/ml staining human LHX3 by Immunohistochemistry in paraffin embedded heart tissue. Cells with positive label (as indicated by the arrows) are myocardial cells. Magnification 400X.

Anti-LHX3 antibody (ab26025) 使用論文

ab26025 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. Cells were lysed in RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM NaF, 1 mM Na3O4, 1 mM PMSF, 1 µg/ml aprotinin, 1 µg/ml leupeptin, 1 µg/ml pepstatin, 1% NP-40, 0.25% sodium deoxycholate). I hop...

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