製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to LHX3
  • 特異性
    Ab14555 recognizes LHX3 and does not appear to crossreact with gsh4 on tissue sections.
  • アプリケーション
    適用あり: ICC/IF, IHC - Wholemount, IHC-P, IHC-Fr, WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Human, Fish
  • 免疫原

    C-terminal portion of mouse LHX3 protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
IHC - Wholemount
IHC-P
IHC-Fr
WB
IP
  • 追加情報
    ICC: 1/200 - 1/500.
    IF: Use at an assay dependent dilution. Use a secondary antibody conjugated to biotin and then streptavidin-conjugated to fluorochrome.
    IHC (whole mounts): 1/4000 - 1/8000. Use in conjunction with MEMFA as fixative.
    IHC-P: 1/250 - 1/1000.
    IHC-Fr: 1/3000 - 1/6000.
    IP: 1/200.
    WB: 1/2500 - 1/5000. Predicted molecular weight: 44 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Acts as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with Pit-1.
    • 関連疾患
      Defects in LHX3 are the cause of pituitary hormone deficiency combined type 3 (CPHD3) [MIM:221750]; also known as combined pituitary hormone deficiency with rigid cervical spine or sensorineural deafness with pituitary dwarfism. CPHD is characterized by a complete deficit in all but one (adrenocorticotropin) anterior pituitary hormone and a rigid cervical spine leading to limited head rotation.
    • 配列類似性
      Contains 1 homeobox DNA-binding domain.
      Contains 2 LIM zinc-binding domains.
    • ドメイン
      The LIM domain specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CPHD 3 antibody
      • CPHD3 antibody
      • DKFZp762A2013 antibody
      • LHX 3 antibody
      • LHX3 antibody
      • LHX3_HUMAN antibody
      • LIM 3 antibody
      • LIM homeobox 3 antibody
      • LIM homeobox gene 3 antibody
      • LIM homeobox protein 3 antibody
      • LIM/homeobox protein Lhx3 antibody
      • LIM/homeodomain protein LHX3 antibody
      • Lim3 antibody
      • M2 LHX3 antibody
      • mLim-3 antibody
      • mLIM3 antibody
      • P LIM antibody
      see all

    Anti-LHX3 antibody (ab14555) 使用論文

    This product has been referenced in:
    • Yan Y  et al. Prdx4 is a compartment-specific H2O2 sensor that regulates neurogenesis by controlling surface expression of GDE2. Nat Commun 6:7006 (2015). IHC . Read more (PubMed: 25943695) »
    • Soundararajan P  et al. Guidance of postural motoneurons requires MAPK/ERK signaling downstream of fibroblast growth factor receptor 1. J Neurosci 30:6595-606 (2010). IHC-Fr ; Chicken . Read more (PubMed: 20463222) »

    See all 4 Publications for this product

    Product Wall

    Thank you for your enquiry. I regret to inform you that there are no details available regarding the characterization of ab14555 in Western blotting. Often it is possible to make suggestions that help resolve problems. We will happily offer technic...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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