The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml.
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
機能Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors (By similarity). Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival.
組織特異性Predominantly expressed in neural tissues, especially in brain. Expression is reduced in low-grade brain tumors and significantly reduced or absent in malignant gliomas. Isoform 1 is absent in the cerebellum and is detectable in the occipital cortex and hippocampus; higher amounts are observed in the parietal and frontal cortices, putamen, and, particularly, in the temporal neocortex, where it is 3.5 times more abundant than in the hippocampus (at protein level). Isoform 3 shows the highest expression in the occipital cortex and the lowest in the hippocampus (at protein level).
関連疾患Defects in LGI1 are the cause of lateral temporal lobe epilepsy autosomal dominant (ADLTE) [MIM:600512]; also known as autosomal dominant partial epilepsy with auditory features (ADPEAF). ADLTE is a form of epilepsy characterized by partial seizures, usually preceded by auditory signs.
Leucine rich glioma-inactivated protein 1 antibody
Leucine-rich glioma-inactivated protein 1 antibody
Anti-Lgi1 antibody 画像
Western blot - Lgi1 antibody (ab69491)
All lanes : Anti-Lgi1 antibody (ab69491) at 1 µg/ml
Lane 1 : mouse brain tissue lysate Lane 2 : mouse brain tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size : 62 kDa Observed band size : 62 kDa Additional bands at : 85 kDa (possible non-specific binding).
Immunohistochemistry (Frozen sections) - Lgi1 antibody (ab69491)This image is courtesy of an anonymous Abreview
ab69491 staining Lgi1 in mouse brain tissue by immunohistochemistry - free floating. The primary antibody was diluted 1/500 and incubated with the sample for 16 hours at 4°C. A biotin conjugated goat anti-rabbit antibody, diluted 1/250, was used as the secondary.