Anti-Lgi1/EPT 抗体 (ab30868)
Key features and details
- Rabbit polyclonal to Lgi1/EPT
- Suitable for: IHC-FoFr, ICC/IF, WB
- Reacts with: Mouse, Rat, Hamster, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Lgi1/EPT antibody
Lgi1/EPT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Lgi1/EPT -
由来種
Rabbit -
アプリケーション
適用あり: IHC-FoFr, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Hamster, Human
交差が予測される動物種: Chicken, Cow -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab30868の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-FoFr | (1) |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 64 kDa).
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特記事項 |
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IHC-FoFr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 64 kDa). |
ターゲット情報
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機能
Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors (By similarity). Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival. -
組織特異性
Predominantly expressed in neural tissues, especially in brain. Expression is reduced in low-grade brain tumors and significantly reduced or absent in malignant gliomas. Isoform 1 is absent in the cerebellum and is detectable in the occipital cortex and hippocampus; higher amounts are observed in the parietal and frontal cortices, putamen, and, particularly, in the temporal neocortex, where it is 3.5 times more abundant than in the hippocampus (at protein level). Isoform 3 shows the highest expression in the occipital cortex and the lowest in the hippocampus (at protein level). -
関連疾患
Defects in LGI1 are the cause of lateral temporal lobe epilepsy autosomal dominant (ADLTE) [MIM:600512]; also known as autosomal dominant partial epilepsy with auditory features (ADPEAF). ADLTE is a form of epilepsy characterized by partial seizures, usually preceded by auditory signs. -
配列類似性
Contains 7 EAR repeats.
Contains 3 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
翻訳後修飾
Glycosylated. -
細胞内局在
Secreted. Cell junction > synapse. Isoform 1 but not isoform 2 is secreted. Isoform 1 is enriched in the Golgi apparatus while isoform 2 accumulates in the endoplasmic reticulum. - Information by UniProt
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参照データベース
- Entrez Gene: 617080 Cow
- Entrez Gene: 9211 Human
- Entrez Gene: 56839 Mouse
- Entrez Gene: 252892 Rat
- Omim: 604619 Human
- SwissProt: O95970 Human
- SwissProt: Q9JIA1 Mouse
- SwissProt: Q8K4Y5 Rat
see all -
別名
- ADLTE antibody
- ADPAEF antibody
- ADPEAF antibody
see all
画像
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All lanes : Anti-Lgi1/EPT antibody (ab30868) at 1 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse brain P0 tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 64 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesGel type: MOPS
Blocking buffer: 2% BSA block
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Anti-Lgi1/EPT antibody (ab30868) at 1/1000 dilution + rat brain tissue lysate at 30 µg
Secondary
HRP-conjugate Goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 64 kDa
Exposure time: 5 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (28)
ab30868 は 28 報の論文で使用されています。
- Hu P et al. A novel LGI1 mutation causing autosomal dominant lateral temporal lobe epilepsy confirmed by a precise knock-in mouse model. CNS Neurosci Ther 28:237-246 (2022). PubMed: 34767694
- Liu F et al. A novel LGI1 missense mutation causes dysfunction in cortical neuronal migration and seizures. Brain Res 1721:146332 (2019). PubMed: 31301272
- Silva J et al. Selective inactivation of LGI1 in neuronal precursor cells leads to cortical dysplasia in mice. Genesis N/A:e23268 (2018). PubMed: 30489685
- Zhou L et al. Celecoxib Ameliorates Seizure Susceptibility in Autosomal Dominant Lateral Temporal Epilepsy. J Neurosci 38:3346-3357 (2018). PubMed: 29491011
- Jang Y et al. LGI1 expression and human brain asymmetry: insights from patients with LGI1-antibody encephalitis. J Neuroinflammation 15:279 (2018). PubMed: 30253786