Anti-LGALS3BP 抗体 [3G8] (ab123921)

製品の概要

  • 製品名Anti-LGALS3BP antibody [3G8]
    LGALS3BP 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3G8] to LGALS3BP
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to internal sequence amino acids 19-300 of Human LGALS3BP produced in E.coli (NP_005558).

  • ポジティブ・コントロール
    • HEK293T cells transfected with pCMV6-ENTRY LGALS3BP cDNA; Human endometrium adenocarcinoma, prostate and bladder carcinoma tissues; COS7 cells transiently transfected by pCMV6-ENTRY LGALS3BP.
  • 特記事項Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)ab123921 is purified from Mouse ascites fluid by affinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名3G8
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab123921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Predicted molecular weight: 65 kDa.
IHC-P 1/150.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

ターゲット情報

  • 機能Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.
  • 組織特異性Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts.
  • 配列類似性Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 1 SRCR domain.
  • 細胞内局在Secreted. Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 90K antibody
    • 90K MAC 2 BP antibody
    • Basement membrane autoantigen p105 antibody
    • BTBD17B antibody
    • Galectin 3 binding protein antibody
    • Galectin-3-binding protein antibody
    • L3 antigen antibody
    • Lectin galactoside binding soluble 3 binding protein antibody
    • Lectin galactoside-binding soluble 3-binding protein antibody
    • LG3BP_HUMAN antibody
    • LGALS3BP antibody
    • M2BP antibody
    • Mac 2 binding protein antibody
    • Mac 2 BP antibody
    • Mac-2 BP antibody
    • Mac-2-binding protein antibody
    • Mac2 BP antibody
    • MAC2BP antibody
    • Serum protein 90K antibody
    • TANGO10B antibody
    • Transport and golgi organization 10 homolog B antibody
    • Tumor associated antigen 90K antibody
    • Tumor-associated antigen 90K antibody
    see all

Anti-LGALS3BP antibody [3G8] 画像

  • All lanes : Anti-LGALS3BP antibody [3G8] (ab123921) at 1/500 dilution

    Lane 1 : HEK293T cell lysate, transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate, transfected with pCMV6-ENTRY LGALS3BP cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 65 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC205039)
  • ab123921 at 1/100 dilution staining LGALS3BP in HEK293T cells transfected with either pCMV6-ENTRY LGALS3BP (Red) or empty vector control plasmid (Blue) by flow cytometry.
  • ab123921 at 1/100 dilution staining LGALS3BP in COS7 cells transiently transfected with pCMV6-ENTRY LGALS3BP by Immunofluorescence.
  • ab123921 at 1/150 dilution staining LGALS3BP in Paraffin-embedded Human Endometrium adenocarcinoma tissue by Immunohistochemistry.
  • ab123921 at 1/150 dilution staining LGALS3BP in Paraffin-embedded Human Prostate tissue by Immunohistochemistry.
  • ab123921 at 1/150 dilution staining LGALS3BP in Paraffin-embedded Human Bladder carcinoma tissue by Immunohistochemistry.

Anti-LGALS3BP antibody [3G8] (ab123921) 使用論文

ab123921 has not yet been referenced specifically in any publications.

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing...

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I have looked through our orders for ab123921 and I cannot find any that use the PO# that you provided. I did find one order from July that could potentially be the correct order, but to confirm could you please either do...

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I am sorry about the issues you have been having with ab123921 and since the protocol tips my colleague provided did not prove to be successful, I will be happy to refund the cost of the antibody for you. To do so co...

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We don't know if TA503455 detects cell surface LGALS3BP or not using FACS as they permeabilize the cells. For your review, I have included the details of the FACS protocol below. The lab only has data using the transfe...

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The concentration of this lot is GR91861-1 is 0.85mg/ml. An appropriate isotype control would be ab91353, Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control. This product is shipped with a concentration of 1mg/ml. http://www.abcam.com/Mouse-IgG...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"