製品の概要

  • 製品名Anti-LexA DNA Binding Region antibody
    LexA DNA Binding Region 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LexA DNA Binding Region
  • 特異性This antibody recognizes E. coli LexA and LexA DBD-tagged fusion proteins.
  • アプリケーション適用あり: WB, IPmore details
  • 免疫原

    Full length protein corresponding to the DNA binding domain (DBD, amino acids 1-222) of E. coli LexA.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/25000 - 1/100000. Detects a band of approximately 130 kDa (predicted molecular weight: 49 kDa). 1/25000 - 1/100000. Detects a band of approximately 130 kDa (predicted molecular weight: 49 kDa). This product has been tested by Western blotting using a LexA-Rb fusion and a LexA-PP2A, beta subunit fusion expressed in budding yeast. The observed molecular weight of a fusion protein will be increased by approximately 24 kDa due to the addition of the LexA DNA binding domain.
IP 1/1.

ターゲット情報

  • 関連性The LexA protein of Escherichia coli is a transcriptional repressor regulating approximately 20 genes, many of which are involved in DNA repair (SOS response). It binds with variable affinity to single, double, or even triple-operators via its amino-terminal domain that contains three alpha-helices spanning residues. This characteristic of LexA protein has been used to make efficient baits for two-hybrid studies.
  • 細胞内局在Nuclear
  • 別名
    • ExrA antibody
    • Lex A antibody
    • LexA antibody
    • LexA repressor antibody
    • Spr antibody
    • Tsl antibody
    • UmuA antibody
    see all

Anti-LexA DNA Binding Region antibody 画像

  • All lanes : Anti-LexA DNA Binding Region antibody (ab50953) at 1/100000 dilution

    Lane 1 : Crude yeast protein extract without a LexA fusion protein
    Lane 2 : Crude yeast protein extract expressing a LexA-Retinoblastoma (Rb) fusion protein

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 49 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

Anti-LexA DNA Binding Region antibody (ab50953) 使用論文

This product has been referenced in:
  • Odsbu I & Skarstad K DNA compaction in the early part of the SOS response is dependent on RecN and RecA. Microbiology 160:872-82 (2014). WB ; Escherichia coli . Read more (PubMed: 24615185) »
  • Erental A  et al. Apoptosis-like death, an extreme SOS response in Escherichia coli. MBio 5:e01426-14 (2014). WB ; Escherichia coli . Read more (PubMed: 25028428) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"