Fusion protein (His-tag) corresponding to Escherichia coli LexA DNA Binding Region aa 1-202. Immunogen is identical for SwissProt: P0A7C2, P0A7C3, P0A7C4
His-tagged DNA binding domain of LexA protein corresponding to aa1-202 Sequence:
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 µg/ml. Predicted molecular weight: 49 kDa.
Detects LexA-pRB in LexA-pRB transfected yeast cells.
Use a concentration of 0.1 µg/ml.
5ug immunoprecipitates LexA-pRB from 500ug LexA-pRB transfected yeast cells.
The LexA protein of Escherichia coli is a transcriptional repressor regulating approximately 20 genes, many of which are involved in DNA repair (SOS response). It binds with variable affinity to single, double, or even triple-operators via its amino-terminal domain that contains three alpha-helices spanning residues. This characteristic of LexA protein has been used to make efficient baits for two-hybrid studies.
Vilaboa N et al. New inhibitor targeting human transcription factor HSF1: effects on the heat shock response and tumor cell survival. Nucleic Acids Res45:5797-5817 (2017).
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Rother S et al. NF-?B-repressing factor phosphorylation regulates transcription elongation via its interactions with 5'?3' exoribonuclease 2 and negative elongation factor. FASEB J30:174-85 (2016).
Read more (PubMed: 26340924) »