製品の概要
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製品名Anti-Leukotriene B4 Receptor 2 antibody
Leukotriene B4 Receptor 2 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Leukotriene B4 Receptor 2
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由来種Rabbit
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アプリケーション適用あり: ICC/IF, WBmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Leukotriene B4 Receptor 2.
(Peptide available as ab94553.)
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ポジティブ･コントロール
- This antibody gave a positive signal in Human brain tissue lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab84600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
ターゲット情報
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機能Low-affinity receptor for leukotrienes including leukotriene B4. Mediates chemotaxis of granulocytes and macrophages. The response is mediated via G-proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTB4 > 12-epi-LTB4 > LTB5 > LTB3.
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組織特異性Widely expressed.
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配列類似性Belongs to the G-protein coupled receptor 1 family.
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細胞内局在Cell membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 56413 Human
- Entrez Gene: 57260 Mouse
- Entrez Gene: 114098 Rat
- Omim: 605773 Human
- SwissProt: Q9NPC1 Human
- SwissProt: Q9JJL9 Mouse
- SwissProt: Q924U0 Rat
- Unigene: 642693 Human
see all -
別名
- BLT2R antibody
- JULF2 antibody
- Leukotriene B4 receptor 2 antibody
see all
画像
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Western blot - Anti-Leukotriene B4 Receptor 2 antibody (ab84600)Anti-Leukotriene B4 Receptor 2 antibody (ab84600) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 15 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Leukotriene B4 Receptor 2 antibody (ab84600)ICC/IF image of ab84600 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84600, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
参考文献
ab84600 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"