製品の概要

  • 製品名
    Anti-Leu Enkephalin antibody
    Leu Enkephalin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Leu Enkephalin
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Leu Enkephalin.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human testis tissue lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab85798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa).

ターゲット情報

  • 関連性
    Enkephalin and enkephalin-like peptides have been detected in many animal species. In mammals these opioid pentapeptides are cleaved from a common precursor molecule which is expressed in a number of cell types, including neurons and endocrine cells associated with the sympathetic nervous system where they are often co-stored with catecholamines. They are known to be endogenous ligands for Sopioid receptors and are known to mediate analgesia at the spinal level.
  • 細胞内局在
    Secreted
  • 参照データベース
  • 別名
    • PENK antibody
    • Proenkephalin A antibody
    • Proenkephalin antibody

画像

  • Anti-Leu Enkephalin antibody (ab85798) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 30 kDa
    Observed band size: 28 kDa (why is the actual band size different from the predicted?)


    Exposure time: 30 seconds


    The band observed at 28 kDa could potentially be a cleaved form of Leu Enkephalin due to the presence of a 24 amino acid signal peptide.
  • ICC/IF image of ab85978 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85798, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.

参考文献

ab85798 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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