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Fusion protein, corresponding to amino acids 1-839 of Human Leptin Receptor fused to the Fc region of human IgG (with IIEGR added at the amino terminus and 6 histidine residues added at the carboxy terminus). Will detect all isoforms of the leptin receptor
Our Abpromise guarantee covers the use of ab2143 in the following tested applications.
|WB||Use at an assay dependent concentration.|
|IHC-P||1/10 - 1/100.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
|Indirect ELISA||Use at an assay dependent concentration.|
ICC/IF image of ab2143 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab2143 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab2143 has not yet been referenced specifically in any publications.