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As per Uniprot database (P48357) OB receptor has 5 isoforms and is highly glycosylated, this means the observed band size will be different than predicted and multiple bands are expected depending upon the isoforms expressed in sample types.
Our Abpromise guarantee covers the use of ab5593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 21151569|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/2000. Detects a band of approximately 100, 125 kDa.Can be blocked with Leptin Receptor peptide (ab5837). Corresponding to the long and short forms (respectively) of leptin receptor OB-R from mouse brain extract. Two smaller bands of approximately 31 and 35 kDa are also detected, which cannot be competed out with immunogenic peptide. Block with 5% milk in TBST at RT. The native leptin receptor is a homodimer held together with disulfide bonds. On a reducing SDS-PAGE, bands may appear as approximately 60 kDa subunits. Further variations in size is possible and is due to glycosylation.|
This image is courtesy of an anonymous abreview.
Blocking agent: 5% Milk for 1 hour at 23°C.
Western blot of both short and long forms of Leptin Receptor on mouse lung tissue extract using ab5593.