製品の概要

  • 製品名Anti-Leptin antibody
    Leptin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Leptin
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Goat, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MHWGTLCGFL WLWPYLFYVQ AVPIQKVQDD TKTLIKTIVT RINDISHTQS) of Human Leptin (NP_000221).

  • ポジティブ・コントロール
    • Human fetal small intestine lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab98090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 19 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • 関連疾患Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • 配列類似性Belongs to the leptin family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ94114 antibody
    • LEP antibody
    • LEP_HUMAN antibody
    • LEPD antibody
    • Leptin (murine obesity homolog) antibody
    • Leptin (obesity homolog, mouse) antibody
    • Leptin antibody
    • Leptin Murine Obesity Homolog antibody
    • Leptin Precursor Obesity Factor antibody
    • OB antibody
    • Obese protein antibody
    • Obese, mouse, homolog of antibody
    • Obesity antibody
    • Obesity factor antibody
    • Obesity homolog mouse antibody
    • Obesity Murine Homolog Leptin antibody
    • OBS antibody
    • OTTHUMP00000212285 antibody
    see all

Anti-Leptin antibody 画像

  • Anti-Leptin antibody (ab98090) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal small intestine lysate at 10 µg

    Predicted band size : 19 kDa
  • ab98090 staining Leptin in placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-Leptin antibody (ab98090) 使用論文

ab98090 has not yet been referenced specifically in any publications.

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With regards to your technical questions:

1.Did you try to block the membrane in 5% BSA instead of 5% milk? Is it 1 hour incubation at RT?
We only use 5% milk for blocking the membrane. Either...

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