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Our Abpromise guarantee covers the use of ab9749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant mouse Leptin (using 100 µl/well antibody solution).|
|WB||Use a concentration of 0.1 - 0.2 µg/ml.|
|IHC-P||Use a concentration of 0.2 µg/ml.|
ab9749 staining Leptin in murine cecum tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in 100mM citric acid. The primary antibody was used at 0.20 ug/ml and incubated with sample at 4°C overnight. A cyanine-3 conjugated donkey polyclonal to rabbit was used as secondary.