製品の概要
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製品名Anti-Leptin antibody
Leptin 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Leptin
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由来種Rabbit
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アプリケーション適用あり: ICC/IF, Inhibition Assay, ICC, IHC-Fr, WB, Flow Cytmore details
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種交差性交差種: Mouse, Rat, Sheep, Human, Pig, Bird
交差が予測される動物種: Goat, Horse, Chicken, Cow, Cat, Dog, Turkey, Chimpanzee, Rhesus monkey
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免疫原
Synthetic peptide corresponding to Mouse Leptin aa 25-44.
Sequence:QKVQDDTKTLIKTIVTRIND
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ポジティブ･コントロール
- ウェスタン・ブロット用ポジティブコントロールRecombinant Human Leptin protein
- WB: 3T3-L1 cell extract IHC: Sheep brain
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab3583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| Inhibition Assay | Use at an assay dependent concentration. | |
| ICC | 1/50 - 1/200. | |
| IHC-Fr | 1/50 - 1/200. | |
| WB | 1/200 - 1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). | |
| Flow Cyt | Use 3-5µl for 106 cells. |
ターゲット情報
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機能May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
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関連疾患Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
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配列類似性Belongs to the leptin family.
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細胞内局在Secreted.
- Information by UniProt
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参照データベース
- Entrez Gene: 493838 Cat
- Entrez Gene: 373955 Chicken
- Entrez Gene: 449638 Chimpanzee
- Entrez Gene: 280836 Cow
- Entrez Gene: 3952 Human
- Entrez Gene: 16846 Mouse
- Entrez Gene: 396832 Pig
- Entrez Gene: 25608 Rat
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別名
- FLJ94114 antibody
- LEP antibody
- LEP_HUMAN antibody
see all
画像
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Western blot - Anti-Leptin antibody (ab3583)ab3583 at a dilution of 1 / 4000 staining leptin in mouse 3T3-L1 cell lysate by Western blot.
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Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (ab3583)ab3583 staining Leptin in 3T3-L1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (2ug/ml in 0.1% BSA) for 3 hours at room temperature. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG (H+L) polyclonal was used as the secondary antibody (1/2000) (Panel a). Nuclei were stained with DAPI (Panel b). F-actin stained with Rhodamine Phalloidin (panel c). Merged images shown in panel d.
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Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (ab3583)ICC/IF image of ab3583 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3583, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (ab3583)This image is courtesy of an Abreview submitted by Steven Johnstoneab3583 staining Leptin in Human mesenchyml stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in 10% horse serum) for 1 hour at 22°C. A FITC-conjugated Mouse anti-rabbit IgG1 monoclonal was used as the secondary antibody (1/100).
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Immunohistochemistry (Frozen sections) - Anti-Leptin antibody (ab3583)Immunohistochemical staining of Leptin in Sheep brain using ab3583.
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Flow Cytometry - Anti-Leptin antibody (ab3583)ab3583 staining Leptin in 3T3-L1 cells by Flow Cytometry. The sample was incubated with the primary antibody (3ug/ml in 2.5% BSA) for 2 hours at room temperature. An Alexa Fluor 488®-conjugated Goat anti-rabbit (1/400) was used as the secondary antibody. Red histogram represents ab3583, pink histogram represents isotype control, purple histogram represents unstained control, green histogram represents no primary antibody control
プロトコール
参考文献
This product has been referenced in:
- Han YC et al. Leptin regulates disc cartilage endplate degeneration and ossification through activation of the MAPK-ERK signalling pathway in vivo and in vitro. J Cell Mol Med N/A:N/A (2018). WB . Read more (PubMed: 29372627) »
- Liu Y et al. HN1L Promotes Triple-Negative Breast Cancer Stem Cells through LEPR-STAT3 Pathway. Stem Cell Reports 10:212-227 (2018). Read more (PubMed: 29249663) »
レビューと Q&A
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