製品の概要

  • 製品名Anti-LEO1 antibody - N-terminal
    LEO1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LEO1 - N-terminal
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the N-terminal region of human LEO1.

  • ポジティブ・コントロール
    • HL60 cell line lysate. 293 cell lysate transiently transfected with the LEO1 gene.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab75721 is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/250. Predicted molecular weight: 75 kDa.
ELISA 1/1000.

ターゲット情報

  • 機能The PAF1 complex is a multifunctional complex. The PAF1 complex interacts with POLR2A. May be involved in both initiation and elongation, histone methylation and RNA processing. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts.
  • 組織特異性Highly expressed in skeletal muscle and heart. Weakly expressed in placenta and liver.
  • 配列類似性Belongs to the LEO1 family.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Gm185 antibody
    • LEO1 antibody
    • LEO1_HUMAN antibody
    • LOC123439 antibody
    • Paf1/RNA polymerase II complex component antibody
    • Replicative senescence down regulated leo1 like protein antibody
    • Replicative senescence down-regulated leo1-like protein antibody
    • Replicative senescence downregulated leo1 like antibody
    • RNA polymerase associated protein LEO1 antibody
    • RNA polymerase-associated protein LEO1 antibody
    • Senescence downregulated leo1 like antibody
    see all

Anti-LEO1 antibody - N-terminal 画像

  • Anti-LEO1 antibody - N-terminal (ab75721) at 1/50 dilution + HL60 cell line lysate at 35 µg

    Predicted band size : 75 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-LEO1 antibody - N-terminal (ab75721) at 1/100 dilution

    Lane 1 : Nontransfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the LEO1 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 75 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

Anti-LEO1 antibody - N-terminal (ab75721) 使用論文

ab75721 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"