製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to LEO1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Chicken, Dog, Xenopus laevis
  • 免疫原

    Synthetic peptide derived from within residues 300 - 400 of Human LEO1.

    (Peptide available as ab27737.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both HeLa and Jurkat whole cell lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab33157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa).

ターゲット情報

画像

  • All lanes : Anti-LEO1 antibody (ab33157) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 75 kDa
    Observed band size: 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 35 kDa, 56 kDa. We are unsure as to the identity of these extra bands.



    ab33157 recognizes a band at 100 kDa that corresponds in size to that seen for LEO1. LEO1 has been shown to migrate at a molecular weight of approximately 100-110 kDa on polyacrylamide gels (see Yart et al., Molecular and Cellular Biology 25(12): 5052-60 and Rozenblatt-Rosen et al., Molecular and Cellular Biology 25(2): 612-20), although it has a predicted molecular weight of 75 kDa.
  • ab33157 staining LEO1 in Mouse ES cells by Immunocytochemistry/ Immunofluorescence. The cells were methanol fixed and blocked in 0.2% Fish scale gelatin for 1 hour at 25°C. Primary antibody was used at 1/100 dilution (0.2% Fish scale gelatin in PBS) and incubated with sample for 20 minutes at 25°C. An Alexa Fluor® 488 conjugated Donkey polyclonal to rabbit IgG was used as secondary at 1/500 dilution. Image shows green color staining with ab33157 and blue with DAPI.   

    See Abreview

  • All lanes : Anti-LEO1 antibody (ab33157) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 75 kDa
    Observed band size: 110 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 52 kDa (possible cross reactivity), 55 kDa (possible cross reactivity)



    ab33157 recognizes a band at 110 kDa that corresponds in size to that seen for LEO1. LEO1 has been shown to migrate at a molecular weight of approximately 100-110 kDa on polyacrylamide gels (see Yart et al., Molecular and Cellular Biology 25(12): 5052-60 and Rozenblatt-Rosen et al., Molecular and Cellular Biology 25(2): 612-20), although it has a predicted molecular weight of 75 kDa.

参考文献

This product has been referenced in:
  • Kim S  et al. Antagonistic regulation of PAF1C and p-TEFb is required for oligodendrocyte differentiation. J Neurosci 32:8201-7 (2012). Read more (PubMed: 22699901) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse ES cells)
Specification
mouse ES cells
Fixative
Methanol
Permeabilization
No
Blocking step
Fish scale gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
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投稿 May 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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