Anti-Legumain 抗体 [EPR14718] (ab183028)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14718] to Legumain
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Legumain antibody [EPR14718]
Legumain 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR14718] to Legumain -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human fetal kidney lysate; JAR, HeLa and 293 cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR14718 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab183028の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 49 kDa.
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特記事項 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 49 kDa. |
ターゲット情報
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機能
Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. -
組織特異性
Ubiquitous. Particularly abundant in kidney, heart and placenta. -
配列類似性
Belongs to the peptidase C13 family. -
翻訳後修飾
Glycosylated. -
細胞内局在
Lysosome. - Information by UniProt
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参照データベース
- Entrez Gene: 5641 Human
- Entrez Gene: 19141 Mouse
- Entrez Gene: 63865 Rat
- Omim: 602620 Human
- SwissProt: Q99538 Human
- SwissProt: O89017 Mouse
- SwissProt: Q9R0J8 Rat
- Unigene: 726036 Human
see all -
別名
- AEP antibody
- Asparaginyl endopeptidase antibody
- cysteine 1 antibody
see all
画像
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All lanes : Anti-Legumain antibody [EPR14718] (ab183028) at 1/10000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : JAR (human placenta choriocarcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 49 kDa
Exposure time: 3 minutesActual band at: 36kDa, 46kDa, 56kDa
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (4)
ab183028 は 4 報の論文で使用されています。
- Chen C et al. Legumain promotes tubular ferroptosis by facilitating chaperone-mediated autophagy of GPX4 in AKI. Cell Death Dis 12:65 (2021). PubMed: 33431801
- Lian J et al. Legumain acts on neuroinflammatory to affect CUS-induced cognitive impairment. Behav Brain Res 376:112219 (2019). PubMed: 31509774
- Wang C et al. Lysophosphatidic acid induces neuronal cell death via activation of asparagine endopeptidase in cerebral ischemia-reperfusion injury. Exp Neurol 306:1-9 (2018). WB ; Rat . PubMed: 29673933
- Završnik J et al. Cystatin C deficiency suppresses tumor growth in a breast cancer model through decreased proliferation of tumor cells. Oncotarget 8:73793-73809 (2017). PubMed: 29088746