製品の概要

  • 製品名Anti-Legumain antibody
    Legumain 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Legumain
  • アプリケーション適用あり: WB, IP, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 100-190 of Human Legumain. This sequence is identical in Mouse and Rat.

  • ポジティブ・コントロール
    • HT29, HeLa, and Mouse Kidney lysates; Human ovary carcinoma tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab125286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
IHC-P Use a concentration of 0.5 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
  • 組織特異性Ubiquitous. Particularly abundant in kidney, heart and placenta.
  • 配列類似性Belongs to the peptidase C13 family.
  • 翻訳後修飾Glycosylated.
  • 細胞内局在Lysosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AEP antibody
    • Asparaginyl endopeptidase antibody
    • cysteine 1 antibody
    • Cysteine protease 1 antibody
    • EC 3.4.22.34 antibody
    • Legumain antibody
    • LGMN antibody
    • LGMN_HUMAN antibody
    • LGMN1 antibody
    • Protease antibody
    • Protease cysteine 1 (legumain) antibody
    • Protease cysteine 1 antibody
    • PRSC1 antibody
    see all

Anti-Legumain antibody 画像

  • All lanes : Anti-Legumain antibody (ab125286) at 1/500 dilution

    Lane 1 : HT29 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Mouse Kidney lysate


    Predicted band size : 49 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab125286, at 1/200 dilution, staining Legumain in formalin-fixed, paraffin-embedded Human ovary carcinoma tissue by Immunohistochemistry.

Anti-Legumain antibody (ab125286) 使用論文

ab125286 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Leech (Hirudo medicinalis) Tissue lysate - whole (Central Nervous System)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 50 µg
Specification Central Nervous System
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Mar 09 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"