The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
See attached protocol.
Legionella pneumophila is a thin, pleomorphic, flagellated Gram-negative bacterium of the genus Legionella. L. pneumophila is the primary human pathogen in this group and is the causative agent of legionellosis or Legionnaires' disease.
L. pneumophila is non-acid-fast, non-sporulating and morphologically a non-capsulated rod-like bacteria. Aerobic and unable to hydrolyse gelatin or produce urease, they are also non-fermentative. L. pneumophila is neither pigmented nor does it autofluoresce. It is oxidase- and catalase-positive and produces beta-lactamase.
While L. pneumophila is categorized as a Gram-negative organism, it stains poorly due to its unique lipopolysaccharide-content in the outer leaflet of the outer cell membrane. The bases for the somatic antigen specificity of these organisms are located on the side-chains of its cell wall. The chemical composition of these side chains both with respect to components as well as arrangement of the different sugars determines the nature of the somatic or O-antigen determinants, which are important means of serologically classifying many Gram-negative bacteria. At least 35 different serovars of L. pneumophila have been described as well as several other species being subdivided into a number of serovars.