Anti-LEFTY2 抗体 [7C5G1H6H10] (ab115224)
Key features and details
- Mouse monoclonal [7C5G1H6H10] to LEFTY2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-LEFTY2 antibody [7C5G1H6H10]
LEFTY2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [7C5G1H6H10] to LEFTY2 -
由来種
Mouse -
特異性
May cross react with the highly homologous LEFTY1. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
A recombinant form of 6X HIS tagged LEFTY2 (Human)
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ポジティブ・コントロール
- Human uterus, myometrium tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
7C5G1H6H10 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115224の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000. Predicted molecular weight: 41 kDa.
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/2000. Predicted molecular weight: 41 kDa. |
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Required for left-right (L-R) asymmetry determination of organ systems in mammals. May play a role in endometrial bleeding. -
組織特異性
Mesenchymal cells of the endometrial stroma. -
関連疾患
Defects in LEFTY2 are the cause of left-right axis malformations (LRAM) [MIM:601877]. The defect includes left pulmonary isomerism, with cardiac anomalies characterized by complete atrioventricular canal defect and hypoplastic left ventricle, and interrupted inferior vena cava. -
配列類似性
Belongs to the TGF-beta family. -
発生段階
Transiently expressed before and during menstrual bleeding. -
翻訳後修飾
The processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 7044 Human
- Entrez Gene: 320202 Mouse
- Entrez Gene: 289316 Rat
- Omim: 601877 Human
- SwissProt: O00292 Human
- SwissProt: P57785 Mouse
- Unigene: 520187 Human
- Unigene: 87078 Mouse
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別名
- EBAF antibody
- Endometrial bleeding associated factor (left right determination factor A transforming growth factor beta superfamily) antibody
- Endometrial bleeding associated factor antibody
see all
画像
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Human uterus, myometrium (formalin fixed, paraffin embedded) stained with ab115224 at 10 ug/ml followed by biotinylated anti Mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen.
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Anti-LEFTY2 antibody [7C5G1H6H10] (ab115224) at 1/2000 dilution + partially purified recombinant 6X His tagged human LEFTY2 at 1000 µg
Predicted band size: 41 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab115224 は論文での使用が確認できていません。