製品の概要

  • 製品名Anti-Lefty antibody [7C5G1H6H10]
    Lefty 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [7C5G1H6H10] to Lefty
  • 特異性This antibody reacts with a 30 kDa protein corresponding to human Lefty. No reactivity occurs against 6X HIS tag.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Recombinant 6X HIS tagged human Lefty.

  • 特記事項Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse Sp2/0 myeloma cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名7C5G1H6H10
  • ミエローマSp2/0
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab34593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 41 kDa).
ELISA 1/5000.

ターゲット情報

  • 関連性During vertebrate embryogenesis, a left right axis is established. Secreted growth factors of the TGF beta family, including gene products derived from nodal, lefty 1 and lefty 2, play crucial roles in establishing left right asymmetries. TGF beta (Transforming growth factor beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF beta actions are directed by ligand induced activation of TGF beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF beta superfamily, inhibits TGF beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF beta receptor. Lefty also inhibits events downstream from R Smad phosphorylation. Lefty provides a repressed state of TGF beta responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (41 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.
  • 細胞内局在Secreted
  • 参照データベース
  • 別名
    • AI450052 antibody
    • Ebaf antibody
    • Left right determination factor 1 antibody
    • Left right determination factor antibody
    • Left right determination factor B antibody
    • left-right determination factor antibody
    • LEFT-RIGHT DETERMINATION FACTOR 1; LEFTY1 antibody
    • left-right determination, factor B antibody
    • LEFT-RIGHT DETERMINATION, FACTOR B; LEFTY B antibody
    • LEFTB antibody
    • LEFTY 1 antibody
    • Lefty antibody
    • lefty-1 antibody
    • LEFTY1 antibody
    • LEFTY1, MOUSE, HOMOLOG OF antibody
    • LEFTYB antibody
    • OTTHUMP00000035572 antibody
    • Protein lefty-1 antibody
    • Protein lefty-B antibody
    • RGD1561867 antibody
    • Stimulated by retinoic acid gene 3 protein antibody
    • STRA3 antibody
    • TGF beta 4 antibody
    • TGF-beta-4 antibody
    • Tgfb4 antibody
    • Transforming growth factor beta 4 antibody
    • Transforming growth factor beta-4 antibody
    see all

Anti-Lefty antibody [7C5G1H6H10] 画像

  • All lanes : Anti-Lefty antibody [7C5G1H6H10] (ab34593) at 1/2000 dilution

    Lane 1 : 1µg 6X His tagged unrelated protein (shows that the antibody does not recognize the epitope tag).
    Lane 2 : 1µg partially purified recombinant 6X his tagged human Lefty

    Secondary
    IRDyeTM800 Goat anti-mouse IgG at 1/1000 dilution

    Predicted band size : 41 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

Anti-Lefty antibody [7C5G1H6H10] (ab34593) 使用論文

ab34593 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"