K562 and Jurkat cell lysate.
This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MEF-1
製品の状態Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報Peptide ELISA: 1/40000 - 1/80000.
WB: 1/200 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 44 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
組織特異性Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
配列類似性Belongs to the TCF/LEF family. Contains 1 HMG box DNA-binding domain.
ドメインProline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
細胞内局在Nucleus. Found in nuclear bodies upon PIASG binding.
ICC/IF image of ab85052 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85052 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - LEF1 antibody (ab85052)
All lanes : Anti-LEF1 antibody (ab85052) at 1/500 dilution
Lane 1 : K562 cell lysate Lane 2 : Jurkat cell lysate
Predicted band size : 44 kDa Observed band size : 44 kDa
Anti-LEF1 antibody (ab85052) 使用論文
has not yet been referenced specifically in any publications.