Anti-LEF1 抗体 (ab74549)
Key features and details
- Rabbit polyclonal to LEF1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-LEF1 antibody
LEF1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LEF1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Chicken, Pig, Monkey -
免疫原
Synthetic peptide corresponding to Human LEF1 aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available asab95919) -
ポジティブ・コントロール
- This antibody gave a positive signal in Jurkat whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74549の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 42, 50 kDa (predicted molecular weight: 44 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 42, 50 kDa (predicted molecular weight: 44 kDa). |
ターゲット情報
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機能
Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. -
組織特異性
Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines. -
配列類似性
Belongs to the TCF/LEF family.
Contains 1 HMG box DNA-binding domain. -
ドメイン
Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors. -
細胞内局在
Nucleus. Found in nuclear bodies upon PIASG binding. - Information by UniProt
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参照データベース
- Entrez Gene: 51176 Human
- Entrez Gene: 16842 Mouse
- Entrez Gene: 161452 Rat
- Omim: 153245 Human
- SwissProt: Q9UJU2 Human
- SwissProt: P27782 Mouse
- SwissProt: Q9QXN1 Rat
- Unigene: 726506 Human
see all -
別名
- DKFZp586H0919 antibody
- FLJ46390 antibody
- LEF 1 antibody
see all
画像
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Anti-LEF1 antibody (ab74549) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 42,50 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 71 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
ab74549 detects a band at 42 and 50 kDa. While this differs to its predicted molecular weight of 44 kDa, this migration has been observed in the literature PMID:17335794.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab74549 は論文での使用が確認できていません。